Home Shop Mn SOD polyclonal antibody

Mn SOD polyclonal antibody

ADI-SOD-110

Datasheet
Manual
SDS
CofA

Already have an account?

SOD (Superoxide dismutase) is responsible for the elimination of cytotoxic active oxygen by catalyzing the dismutation of the superoxide radical to oxygen and hydrogen peroxide. There are three SOD isoenzymes in mammalian cells, they are: EC SOD (extracellular SOD), Cu/Zn SOD (copper and zinc-containing SOD) and Mn SOD (manganese-containing SOD).

Shipping: Available products typically ship within 24/48h, via priority shipping.

Do you need support? Contact Customer Service or Technical Support.

Online Account
Access or Create Your Account

This antibody is covered by our Worry-Free Guarantee.

Mn SOD polyclonal antibody Western blot — Western blot analysis of Mn SOD: Lane 1: MW Marker, Lane 2: HeLa Cell Lysate, Lane 3: Mouse Brain Tissue Extract, Lane 4: Rat Brain Tissue Extract

Mn SOD polyclonal antibody Immunohistochemistry — Immunohistochemistry analysis of human skeletal muscle tissue stained with Mn SOD, pAb at 10µg/ml.

ADI-SOD-110 Mn SOD polyclonal antibody Western Blot Mouse 1 — PGC1 α1 levels increase in the soleus in trained mice and in transfected C2C12 cells upon transfection with pCMV-Entry-HSPD1 vector.(A) representative western blots of soleus and relative expression levels (bars) of PGC1 α1 (113 kDa), 4 HNE (55 kDa), Mn SOD (25 kDa), p-AMPKα (63 kDa), AMPKα1 (63 kDa), AMPKα2 (63 kDa), TFAM (30 kDa) in soleus of sedentary (SED45, open bar, n = 8) and trained (TR45, shaded bar, n = 8) mice at 45 days. 80 μg of proteins were loaded in each lane; GAPDH (37 kDa) was used as the loading control. Data are presented as the means ± SD. ∂ significantly different from TR45 mice (P < 0.001). AU: Arbitrary Unit. (B) representative western blots of C2C12 cells and relative levels (bars) of PGC1 α1 (113 kDa), 4 HNE (55 kDa), Mn SOD (25 kDa), p-AMPKα (63 kDa), AMPKα1 (63 kDa), AMPKα2 (63 kDa), TFAM (30 kDa) in C2C12 myoblast transfected with pCMV6-Entry-HSPD1 vector (pcDNA3.1 was used as a negative control). 80 μg of proteins were loaded in each lane; GAPDH (37 kDa) was used as the loading control and TR45 was used as positive control. Data are presented as the means ± SD. #significantly different from pCMV6-Entry-HSPD1 (P < 0.0001). AU: Arbitrary Unit. *significant results. (C) relative expression levels of 4 HNE, p-AMPKα, p-AMPKα/(AMPKα1 + AMPKα2) in the soleus. Open bars, sedentary mice; shaded bars, trained mice. AU: Arbitrary Unit.

Image collected and cropped by CiteAb under a CC-BY license from the following publication: Skeletal muscle Heat shock protein 60 increases after endurance training and induces peroxisome proliferator-activated receptor gamma coactivator 1 α1 expression. Sci Rep (2016)

Product Details

Alternative Name	Manganese superoxide dismutase
Application	IHC, WB
Application Notes	Detects a band of ~25kDa by Western blot.
Formulation	Liquid. In PBS containing 50% glycerol and 0.09% sodium azide.
Gene/Protein Identifier	NP_000627 (RefSeq)
Host	Rabbit
Immunogen	Human Mn SOD protein.
Purity Detail	Protein A affinity purified.
Recommendation Dilutions/Conditions	Western Blot (1:1,000, colorimetric)Suggested dilutions/conditions may not be available for all applications.Optimal conditions must be determined individually for each application.
Source	Purified from rabbit serum.
Species Reactivity	Bovine, Dog, Guinea pig, Hamster, Human, Monkey, Mouse, Rabbit, Rat, Sheep, Xenopus
UniProt ID	P04179
Worry-free Guarantee	This antibody is covered by our Worry-Free Guarantee.

Handling & Storage

Handling	Avoid freeze/thaw cycles.
Long Term Storage	-20°C
Shipping	Blue Ice

Regulatory Status	RUO – Research Use Only

Reducing Dietary Protein Enhances the Antitumor Effects of Chemotherapy through Immune-Mediated Mechanisms: Mulkeen, S. C., Saha, S., et al.; Mol. Cancer Ther. 24, 1099 (2025), Application(s): Western Blot, Abstract
Rosmarinic Acid Inhibits Ultraviolet B-Mediated Oxidative Damage via the AKT/ERK-NRF2-GSH Pathway In Vitro and In Vivo: M. J. Piao, et al.; Biomol. Ther. 32, 84 (2024), Abstract
Liver iron stores and effectors of ferroptosis are dependent on age and sex: Bloomer, S. A., Wagner, B. A., et al.; Exp. Physiol. 109, 2046 (2024), Abstract
Differential effects of diphenyl diselenide (PhSe)2on mitochondria-related pathways depending on the cellular energy status in Bovine Vascular Endothelial Cells: Galant, L. S., Doblado, L., et al.; bioRxiv , (2024)
Mitochondrial cardiolipin metabolism controlled by tafazzin enables ferroptosis: Wohlfarter, Y., Hagenbuchner, J., et al.; bioRxiv , (2024)
Interferon Gamma Enhances Cytoprotective Pathways via Nrf2 and MnSOD Induction in Friedreich’s Ataxia Cells.: Luffarelli, R., Panarello, L., et al.; Int. J. Mol. Sci. 24, (2023), Application(s): WB / Reactant(s): Human, Abstract
Extracellular Superoxide Dismutase Attenuates Hepatic Oxidative Stress in Nonalcoholic Fatty Liver Disease through the Adenosine Monophosphate-Activated Protein Kinase Activation: H. Nam, et al.; Antioxidants 12, 2040 (2023), Abstract
Astrocytic Nrf2 Mediates the Neuroprotective and Anti-Inflammatory Effects of Nootkatone in an MPTP-Induced Parkinson’s Disease Mouse Model: J.E. Park, et al.; Antioxidants 12, 1999 (2023), Abstract
Synergistic Effects of Vitis vinifera L. and Centella asiatica against CCl4-Induced Liver Injury in Mice.: Munakarmi, S., Gurau, Y., et al.; Int. J. Mol. Sci. 24, (2023), Reactant(s): Mouse, Abstract
Anti-inflammatory reprogramming of microglia cells by metabolic modulators to counteract neurodegeneration; a new role for Ranolazine: Piano, I., Votta, A., et al.; Sci. Rep. 13, 20138 (2023), Abstract