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αB-Crystallin polyclonal antibody

ADI-SPA-223

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Alpha-crystallins, which are part of the small Heat shock family members, are major water-soluble proteins present in the lens of the mammalian eye. Phosphorylation of serine residues which occurs during development and in response to stress, is intimately linked with its function. Chaperone activity requires, and is modulated by, oligomerization and is limited to binding unfolded intermediates to prevent irreversible aggregation.

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αB-Crystallin polyclonal antibody Western blot — Western blot analysis of αB-Crystallin: Lane 1: MW marker, Lane 2:α-Crystallin (bovine), (native) (Prod No. ADI-SPP-225), Lane 3: αA-Crystallin (bovine), (native) (Prod No. ADI-SPP-226), Lane 4:αB-Crystallin (bovine), (native) (Prod No. ADI-SPP-227), Lane 5: β-Crystallin (bovine), (native) (Prod No. ADI-SPP-235)

αB-Crystallin polyclonal antibody Immunohistochemistry — Immunohistochemistry analysis of human brain, cortex tissue stained with αB-Crystallin, pAb at 10µg/ml.

Product Details

Alternative Name	CRYAB, HSPB5
Application	IF, IHC (PS), WB
Application Notes	Detects a band of ~20kDa by Western blot.
Formulation	Liquid. In PBS, pH 7.2, containing 50% glycerol and 0.09% sodium azide.
GenBank ID	M28638
Host	Rabbit
Immunogen	Synthetic peptide corresponding to the sequence near the C-terminus of human αB-Crystallin. The sequence is completely conserved in rat, rabbit, and bovine.
Purity Detail	Protein A affinity purified.
Recommendation Dilutions/Conditions	Western Blot (1:1,000, colorimetric)Suggested dilutions/conditions may not be available for all applications.Optimal conditions must be determined individually for each application.
Source	Purified from rabbit serum.
Species Reactivity	Bovine, Human, Mouse, Porcine, Rat
UniProt ID	P02511
Worry-free Guarantee	This antibody is covered by our Worry-Free Guarantee.

Handling & Storage

Long Term Storage	-20°C
Shipping	Blue Ice

Regulatory Status	RUO – Research Use Only

Phosphorylation of CRYAB induces a condensatopathy to worsen post-myocardial infarction left ventricular remodeling: Islam, M., Rawnsley, D. R., et al.; J. Clin. Invest. , e163730 (2025), Abstract
Gluk4-containing kainate receptors regulate synaptic communication in the motor cortex and reduce axon degeneration in adult mice: Ricci, R., Fletcher, J. L., et al.; bioRxiv , (2024), Reactant(s): Mouse
Tumor suppressor p53 regulates heat shock factor 1 protein degradation in Huntington’s disease: Mansky, R. H., Greguske, E. A., et al.; Cell Rep. 42, 112198 (2023), Abstract
Lipid transfer proteins and a PI 4-kinase initiate nuclear phosphoinositide signaling: Carrillo, N. D., Chen, M., et al.; bioRxiv , (2023)
Eye Lens Organoids Made Simple: Characterization of a New Three-Dimensional Organoid Model for Lens Development and Pathology.: Duot, M., Viel, R., et al.; Cells 12, (2023), Application(s): IHC, Abstract
Restored autophagy is protective against PAK3-induced cardiac dysfunction.: Ruiz-Velasco, A., Raja, R., et al.; iScience 26, 106970 (2023), Application(s): IHC, Abstract
Loss of function variants in DNAJB4 cause a myopathy with early respiratory failure.: Weihl, C., Töpf, A., et al.; Acta Neuropathol. 145, 127 (2023), Application(s): IHC-IF, Abstract
Loss of function variants in DNAJB4 cause a myopathy with early respiratory failure: Weihl, C. C., Töpf, A., et al.; bioRxiv , (2022)
Crystallins Play a Crucial Role in Glaucoma and Promote Neuronal Cell Survival in an In Vitro Model Through Modulating Müller Cell Secretion: H. Liu, et al.; Invest. Ophthalmol. Vis. Sci. 63, 3 (2022), Application(s): IHC / Reactant(s) Rat, Abstract
Oxidative stress differentially impacts apical and basolateral secretion of angiogenic factors from human iPSC-derived retinal pigment epithelium cells.: Chen, L., Perera, N. D., et al.; Sci. Rep. 12, 12694 (2022), Application(s): WB, Abstract