Zymolyase® from Arthrobacter luteus, min. 100 U/mg

Zymolyase®, produced by a submerged culture of Arthrobacter luteus, has strong lytic activity against living yeast cell walls to produce protoplast or spheroplast of various strains of yeast cells. This enzyme is prepared by ammonium sulfate precipitation and further purified by affinity chromatography. The essential enzyme activity for the lysis of yeast cells is β-1,3-glucan laminaripentaohydrolase. It hydrolyzes linear glucose polymers with β-1,3-linkages and releases specifically laminaripentaose as the main and minimum product unit. Lytic activity varies depending on yeast strain, growth stage of yeast, or cultural conditions.