ZediXclusive TG6-ab ELISA (IgA)

The present Enzyme-Linked Immuno Sorbent Assay (ELISA) is intended for the quantitative or qualitative determination of IgA antibodies in human serum, directed against TG6. The immobilised antigen is a highly purified preparation of human recombinant TG6. The test is fast (incubation time 30 / 30 / 30 minutes) and flexible (divisible solid phase, ready-to-use reagents). Six calibrators allow quantitative measurements; a negative and a positive control check the assay performance.

Assay principle
The wells of the solid phase are coated with TG6.
1^st reaction: TG6-specific antibodies present in the sample bind to the immobilised antigen, forming the antigen-antibody complex.
2^nd reaction: A second antibody, directed at human IgA antibodies and labeled with horse-radish peroxidase (HRP), binds to the complex.
3^rd reaction: The enzyme-labelled complex converts a substrate into a blue product. Samples containing IgA antibodies against TG6 develop the blue colour, whereas samples without these antibodies remain colourless.