ZediXcite 360/460 Fluorogenic TG2-Assay Kit

TISSUE TRANSGLUTAMINASE (TG2, transglutaminase 2) is a multifunctional enzyme best known for its crosslinking activity, but it can also cleave isopeptide bonds. This feature is used to provide an easy to handle, robust, sensitive, and precise fluorogenic assay to measure TG2 activity. The assay is suitable for research purposes, drug discovery programs, and quality assurance.
TISSUE TRANSGLUTAMINASE cleaves a dark quencher molecule from the side chain of a TG2 specific peptide (SUBSTRATE REAGENT SR). The TG2-substrate complex is cleaved by the incorporation of a glycine methyl ester molecule. Subsequently, the fluorescence of an N-terminal coupled fluorophore increases and can be continuously monitored (excitation wavelength 360 nm; emission wavelength 460 nm).