Zebrafish TRβ Reporter Assay System

INDIGO’s Zebrafish Thyroid Hormone Receptor Beta Reporter Assay System utilizes proprietary human cells engineered to provide constitutive, high-level expression of Zebrafish (Danio rerio) Thyroid Hormone Receptor Beta (nr1a2), a ligand-dependent transcription factor.

INDIGO’s Reporter Cells include the luciferase reporter gene functionally linked to a TRβ-responsive promoter. Thus, quantifying changes in luciferase expression in the test sample-treated reporter cells provides a sensitive surrogate measure of changes in zTRβ activity. The principal application of this assay is in the screening of test samples to quantify any functional bioactivity that they may exert against zebrafish AR. In particular, zebrafish reporter assays are frequently used in the monitoring of environmental samples for the presence of biohazardous chemical pollutants, such as endocrine disruptors.