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Z-YVAD-AFC

ALX-260-035

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Caspase-1 substrate

Fluorogenic substrate for caspase-1. AFC has an excitation maximum of 400nm and an emission maximum of 505nm. This product is similar to Ac-YVAD-AMC, Prod. No. ALX-260-024, but the AFC fluorophore has a greater Stokes’ shift upon cleavage and the N-terminal is blocked by a benzyloxycarbonyl moiety, which increases cell permeability.

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Product Details

Alternative Name	Caspase-1 substrate (fluorogenic)
Appearance	White to off-white powder.
CAS	201608-13-1
Formula	C₃₉H₄₀N₅O₁₁F₃
MW	811.8
Purity	≥95% (HPLC).Single spot (TLC)
Sequence	Z-Tyr-Val-Ala-Asp-AFC (AFC = 7-Amido-4-trifluoromethylcoumarin)
Solubility	Soluble in DMSO, DMF or methanol; insoluble in water.

Handling & Storage

Use/Stability	As indicated on product label or CoA when stored as recommended. DMF or DMSO stock solution highly stable at -20°C.
Handling	Protect from light.
Long Term Storage	-20°C
Shipping	Ambient Temperature

Regulatory Status	RUO – Research Use Only

The Crohn’s Disease Risk Factor IRGM Limits NLRP3 Inflammasome Activation by Impeding Its Assembly and by Mediating Its Selective Autophagy: Mehto, S., Jena, K. K., et al.; Mol. Cell 73, 429 (2019), Abstract
Laquinimod decreases Bax expression and reduces caspase-6 activation in neurons: D. Ehrnhoefer, et al.; Exp. Neurol. 283A, 121 (2016), Application(s): Western blot, Abstract
Activity-Based Proteomic Profiling of Deubiquitinating Enzymes in Salmonella-Infected Macrophages Leads to Identification of Putative Function of UCH-L5 in Inflammasome Regulation: E. Kummari, et al.; PLoS One 10, e0135531 (2015), Application(s): Measurement of caspase-1, Abstract — Full Text
Activation of multiple interleukin-1beta converting enzyme homologues in cytosol and nuclei of HL-60 cells during etoposide-induced apoptosis: L.M. Martins, et al.; J. Biol. Chem. 272, 7421 (1997), Abstract — Full Text
BAX-induced cell death may not require interleukin 1 beta-converting enzyme-like proteases: J. Xiang, et al.; PNAS 93, 14559 (1996), Abstract
Crystal structure of the cysteine protease interleukin-1 beta- converting enzyme: a (p20/p10)2 homodimer: N.P. Walker, et al.; Cell 78, 343 (1994), Abstract
Peptidic p-nitroanilide substrates of interleukin-1 β-converting enzyme: L.A. Reiter; Int. J. Pept. Protein Res. 43, 87 (1994), Abstract
Interleukin-1 beta converting enzyme: N.A. Thornberry; Meth. Enzymol. 244, 615 (1994), Abstract
Interleukin 1 beta (IL-1 beta) processing in murine macrophages requires a structurally conserved homologue of human IL-1 beta converting enzyme: S.M. Molineaux, et al.; PNAS 90, 1809 (1993), Abstract
A novel heterodimeric cysteine protease is required for interleukin-1 β processing in monocytes: N.A. Thornberry, et al.; Nature 356, 768 (1992), Abstract