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Pan-Caspase Inhibitor. Z-VAD-FMK acts as an effective irreversible caspase inhibitor with no cytotoxic effects and, therefore, is a useful tool for studying caspase activity.
- Irreversible inhibitor
- Cell-permeable
- High purity peptide
- Non-cytotoxic
Irreversible, cell permeable broad-spectrum caspase inhibitor.
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Product Details
| Alternative Name |
Caspase family inhibitor (fluoromethylketone), Z-Val-Ala-DL-Asp-FMK |
|---|---|
| Appearance |
Solid |
| Application Notes |
Can be used as a broad spectrum caspase inhibitor in conjunction with apoptosis detection assays. |
| CAS |
220644-02-0 |
| Formula |
C21H28FN3O7 |
| MW |
453.5 |
| Peptide Content |
77-97% |
| Purity |
≥95% (HPLC) |
| Sequence |
Z-Val-Ala-Asp-fluoromethylketone |
| Solubility |
Soluble in DMSO or acetonitrile (10mg/ml). |
Handling & Storage
| Use/Stability |
As indicated on product label or CoA when stored as recommended. Keep sealed after removing from the freezer until its temperature equilibrates with room temperature. Dissolve only the amount needed at one time because of the possibility of decomposition in solution. |
|---|---|
| Handling |
After reconstitution, prepare aliquots and store at -20°C. |
| Long Term Storage |
-20°C |
| Shipping |
Ambient Temperature |
| Regulatory Status |
RUO – Research Use Only |
|---|
- Activation of lysosomal iron triggers ferroptosis in cancer: Cañeque, T., Baron, L., et al.; Nature 642, 492 (2025), Abstract
- Dipeptidyl Peptidase 9 in Human Leukocytes: Novel Inhibitors Induce Caspase-1- and Gasdermin-Dependent Cell Death: De Loose, J., Mertens, K., et al.; Eur. J. Immunol. 55, e51526 (2025), Abstract
- Protocol for CRISPR-Cas9-mediated induction of KMT2A rearrangements in cell line and umbilical cord blood hematopoietic stem and progenitor cells: Benz, T., Larghero, P., et al.; STAR Protoc. 6, 103481 (2025), Abstract
- A Potent Inhibitor of Caspase‑8 Based on the IL-18 Tetrapeptide Sequence Reveals Shared Specificities between Inflammatory and Apoptotic Initiator Caspases: C.M. Bourne, et al.; bioRxiv , 02.23.639785 (2025), Abstract — Full Text
- Activation of lysosomal iron triggers ferroptosis in cancer: R. Rodriguez, et al.; Res. Sq. , (2024), Abstract
- Activation of the NLRP1B inflammasome by caspase-8: Meade, J. J., Stuart, S., et al.; Commun. Biol. 7, 1164 (2024), Abstract
- Advanced age and female sex protect cerebral arteries from mitochondrial depolarization and apoptosis during acute oxidative stress: Norton, C. E., Shaw, R. L., et al.; Aging Cell 23, e14110 (2024), Reactant(s): Mouse, Abstract
- Distinct mechanisms of type 3 secretion system recognition control LTB4 synthesis in neutrophils and macrophages: Brady, A., Mora Martinez, L. C., et al.; PLoS Pathog. 20, e1012651 (2024), Abstract
- Distinct Mechanisms of Type 3 Secretion System Recognition Control LTB4Synthesis in Neutrophils versus Macrophages: Brady, A., Mora-Martinez, L. C., et al.; bioRxiv , (2024)
- Epigenetic Priming by Hypomethylation Enhances the Immunogenic Potential of Tolinapant in T-cell Lymphoma: Ward, G., Zhang, Z., et al.; Cancer Res. Commun. 4, 1441 (2024), Abstract
- Glial state changes and neuroinflammatory RIPK1 signaling are a key feature of ALS pathogenesis: Zelic, M., Blazier, A., et al.; bioRxiv , (2024)
- Necroptosis induced by ruthenium (II) complexes as mitochondrial disruptors: Gonçalves, J., Amaral, J. D., et al.; Cell Death Discov. 10, 261 (2024), Abstract
- RIPK1 protects naive and regulatory T cells from TNFR1-induced apoptosis: Huysentruyt, J., Steels, W., et al.; Cell Death Differ. 31, 820 (2024), Abstract
- The novel drug candidate S2/IAPinh improves survival in models of pancreatic and ovarian cancer: Hagi, T., Vangveravong, S., et al.; Sci. Rep. 14, 6373 (2024), Abstract
- Tumor suppressor Par-4 activates autophagy-dependent ferroptosis: Subburayan, K., Thayyullathil, F., et al.; Commun. Biol. 7, 732 (2024), Abstract
- LAMP3 transfer via extracellular particles induces apoptosis in Sjögren’s disease: T. Tanaka, et al.; Sci. Rep. 13, 2595 (2023), Abstract
- 14-3-3 proteins regulate cullin 7-mediated Eag1 degradation: C.H. Hsieh, et al.; Cell Biosci. 13, 18 (2023), Abstract
- Inflammasome-independent NLRP3 function enforces ATM activity in response to genotoxic stress: M.B. Wachte, et al.; Life Sci. Alliance 6, e202201494 (2023), Abstract
- Monocarbonyl Analogs of Curcumin with Potential to Treat Colorectal Cancer: M. Clariano, et al.; Chem. Biodivers. 20, 202300222 (2023), Abstract
- Solanum nigrum Fruit Extract Modulates Immune System Activity of Mealworm Beetle, Tenebrio molitor L: A. Urbański, et al.; Toxins 15, 68 (2023), Abstract
- Targeting oxeiptosis-mediated tumor suppression: a novel approach to treat colorectal cancers by sanguinarine: S. Pallichankandy, et al.; Cell Death Discov. 9, 94 (2023), Abstract
- Myeloid OTULIN deficiency couples RIPK3-dependent cell death to Nlrp3 inflammasome activation and IL-1β secretion: M.G. Doglio, et al.; Sci. Immunol. 8, eadf4404 (2023), Abstract
- Integrated chemical and genetic screens unveil FSP1 mechanisms of ferroptosis regulation: T. Nakamura, et al.; Nat. Struct. Mol. Biol. 30, 1806 (2023), Abstract
- Triggering endogenous Z-RNA sensing for anti-tumor therapy through ZBP1-dependent necroptosis: T. Yang, et al.; Cell Rep. 42, 113377 (2023), Abstract
- Defective quality control autophagy in Hyperhomocysteinemia promotes ER stress and consequent neuronal apoptosis through proteotoxicity: B. Kaur, et al.; Cell Commun. Signal. 21, 258 (2023), Abstract
- Synergistic anticancer activity of combined ATR and ribonucleotide reductase inhibition in Ewing’s sarcoma cells: M.J. Sturm, et al.; J. Cancer Res. Clin. Oncol. 149, 8605 (2023), Abstract
- Phase separation of FSP1 promotes ferroptosis: T. Nakamura, et al.; Nature 619, 371 (2023), Abstract
- Acylation of MLKL impacts its function in necroptosis: Pradhan, A. J., Chitkara, S., et al.; bioRxiv , (2023)
- Catalytic inhibitor of Protein Phosphatase 5 activates the extrinsic apoptotic pathway by disrupting complex II in kidney cancer: Ahanin, E. F., Sager, R. A., et al.; Cell Chem. Biol. 30, 1223 (2023), Reactant(s): Human, Abstract
- Effect of erythrophagocytosis-induced ferroptosis during angiogenesis in atherosclerotic plaques: Puylaert, P., Roth, L., et al.; Angiogenesis 26, 505 (2023), Abstract
- Macrophage innate immune responses delineate between defective translocon assemblies produced by Yersinia pseudotuberculosis YopD mutants: Farag, S. I., Francis, M. K., et al.; Virulence 14, 2249790 (2023), Abstract
- SREBP activation contributes to fatty acid accumulations in necroptosis: Lu, D., Parisi, L. R., et al.; RSC chem. biol. 4, 310 (2023), Abstract
- Apoptosis as Driver of Therapy-Induced Cancer Repopulation and Acquired Cell-Resistance (CRAC): A Simple In Vitro Model of Phoenix Rising in Prostate Cancer: F. Corsi, et al.; Int. J. Mol. Sci. 23, 1152 (2022), Abstract
- Exosomal p38 Mitogen-activated Protein Kinase Promotes Tumour Repopulation in TP53-mutated Bile Duct Cancer Cells: M. Osawa, et al.; Anticancer Res. 42, 745 (2022), Abstract
- Protection of Quiescence and Longevity of IgG Memory B Cells by Mitochondrial Autophagy: S. Kodali, et al.; J. Immunol. 208, 1085 (2022), Abstract
- The combination of TRAIL and the Smac mimetic LCL-161 induces an irreversible phenotypic change of MCF-7 breast cancer cells: V. Granqvist, et al.; Exp. Mol. Pathol. 125, 104739 (2022), Abstract
- Blockade of caspase cascade overcomes malaria-associated acute respiratory distress syndrome in mice: M.K. Sercundes, et al.; Cell Death Dis. 13, 144 (2022), Abstract
- A non-canonical vitamin K cycle is a potent ferroptosis suppressor: E. Mishima, et al.; Nature 608, 778 (2022), Abstract
- Caspase cleavage and nuclear retention of the energy sensor AMPK-α1 during apoptosis: A.R. Cheratta, et al.; Cell Rep. 39, 110761 (2022), Abstract
- Hepatocyte Bcl-3 protects from death-receptor mediated apoptosis and subsequent acute liver failure: N. Gehrke, et al.; Cell Death Dis. 13, 510 (2022), Abstract
- MDM2 binds and ubiquitinates PARP1 to enhance DNA replication fork progression: C. Giansanti, et al.; Cell Rep. 39, 110879 (2022), Abstract
- Dasatinib and interferon alpha synergistically induce pyroptosis-like cell death in philadelphia chromosome positive acute lymphoblastic leukemia: Y. Dai, et al.; Am. J. Cancer Res. 12, 2817 (2022), Abstract
- The Impact of RIPK1 Kinase Inhibition on Atherogenesis: A Genetic and a Pharmacological Approach: P. Puylaert, et al.; Biomedicines 10, 1016 (2022), Abstract
- Autophagy promotes cell survival by maintaining NAD levels: T. Kataura, et al.; Dev. Cell 57, 2584 (2022), Abstract
- Induction of Breast Cancer Cell Apoptosis by TRAIL and Smac Mimetics: Involvement of RIP1 and cFLIP: C. Holmgren, et al.; Curr. Issues Mol. Biol. 44, 4803 (2022), Abstract
- Endothelial cells promote smooth muscle cell resilience to H2 O2 -induced cell death in mouse cerebral arteries: Norton, C. E., Shaw, R. L., et al.; Acta Physiol. (Oxf.) 235, e13819 (2022), Reactant(s): Mouse, Abstract
- Heightened NLRP3 inflammasome activation is associated with aging and CMML diseases severity: Andina, N., de Meuron, L., et al.; bioRxiv , (2022)
- LAMP3 inhibits autophagy and contributes to cell death by lysosomal membrane permeabilization: Tanaka, T., Warner, B. M., et al.; Autophagy 18, 1629 (2022), Abstract
- SREBP1 activation contributes to fatty acid accumulations in necroptosis: Lu, D., Parisi, L. R., et al.; bioRxiv , (2022)
- Targeting plasma membrane and mitochondrial instability in breast cancer cells and breast epithelial to mesenchymal transition-model cells by adamantyl diaza-crown ether ZG613: Ester, K., Mioč, M., et al.; bioRxiv , (2022)
- Potential IFNγ Modulation of Inflammasome Pathway in Chlamydia trachomatis Infected Synovial Cells: S. Filardo, et al.; Life (Basel) 11, 1359 (2021), Abstract
- Quantitative differences between cyclin-dependent kinases underlie the unique functions of CDK1 in human cells: H.W. Lau, et al.; Cell Rep. 37, 109808 (2021), Abstract
- Chaperon-mediated autophagy can regulate diquat-induced apoptosis by inhibiting α-synuclein accumulation cooperatively with macroautophagy: H.J. Kim & H.C. Koh; Food Chem. Toxicol. 158, 112706 (2021), Abstract
- Autophagy-competent mitochondrial translation elongation factor TUFM inhibits caspase-8-mediated apoptosis: C.Y. Choi, et al.; Cell Death Differ. , (2021), Abstract
- Caspase-9 Activation of Procaspase-3 but Not Procaspase-6 Is Based on the Local Context of Cleavage Site Motifs and on Sequence: I.V. Soni & J.A. Hardy; Biochemistry 60, 2824 (2021), Abstract
- Protective role of cytoplasmic p21Cip1/Waf1 in apoptosis of CDK4/6 inhibitor-induced senescence in breast cancer cells: I.D. Kartika, et al.; Cancer Med. 10, 8988 (2021), Abstract
- Elesclomol-induced increase of mitochondrial reactive oxygen species impairs glioblastoma stem-like cell survival and tumor growth: M. Buccarelli, et al.; J. Exp. Clin. Cancer Res. 40, 228 (2021), Abstract
- Rapid Release of Interleukin-1β from Human Platelets Is Independent of NLRP3 and Caspase: G.J. Pennings, et al.; Thromb. Haemost. , (2021), Abstract
- Anti-tumor effects of an ID antagonist with no observed acquired resistance: P.M. Wojnarowicz, et al.; NPJ Breast Cancer 7, 41523 (2021), Abstract
- Induction of interferon-β and interferon signaling by TRAIL and Smac mimetics via caspase-8 in breast cancer cells: V. Granqvist, et al.; PLoS One 16, 248175 (2021), Abstract — Full Text
- Betulin, a Newly Characterized Compound in Acacia auriculiformis Bark, Is a Multi-Target Protein Kinase Inhibitor: A.A. Ahmadu, et al.; Molecules 26, 4599 (2021), Abstract
- Alzheimer’s-associated PU.1 expression levels regulate microglial inflammatory response: Pimenova, A. A., Herbinet, M., et al.; Neurobiol. Dis. 148, 105217 (2021), Abstract
- Caspase 3/GSDME-dependent pyroptosis contributes to chemotherapy drug-induced nephrotoxicity: Shen, X., Wang, H., et al.; Cell Death Dis. 12, 186 (2021), Abstract
- Embryo size regulates the timing and mechanism of pluripotent tissue morphogenesis: Orietti, L. C., Rosa, V. S., et al.; Stem Cell Reports 16, 1182 (2021), Reactant(s): Mouse, Abstract
- Evolutionary loss of inflammasomes in the Carnivora and implications for the carriage of zoonotic infections: Digby, Z., Tourlomousis, P., et al.; Cell Rep. 36, 109614 (2021), Abstract
- Loss of vacuolar-type H+-ATPase induces caspase-independent necrosis-like death of hair cells in zebrafish neuromasts: Santra, P., Amack, J. D., et al.; Dis. Model. Mech. 14, (2021), Reactant(s): Zebrafish, Abstract
- OTUB1 prevents lethal hepatocyte necroptosis through stabilization of c-IAP1 during murine liver inflammation: Koschel, J., Nishanth, G., et al.; Cell Death Differ. 28, 2257 (2021), Abstract
- OTULIN inhibits RIPK1-mediated keratinocyte necroptosis to prevent skin inflammation in mice: Schünke, H., Göbel, U., et al.; Nat. Commun. 12, 5912 (2021), Reactant(s): Mouse, Abstract
- Protein acylation by saturated very long chain fatty acids and endocytosis are involved in necroptosis: Pradhan, A. J., Lu, D., et al.; Cell Chem. Biol. 28, 1298 (2021), Abstract
- RIPK1 activation mediates neuroinflammation and disease progression in multiple sclerosis: Zelic, M., Pontarelli, F., et al.; Cell Rep. 35, 109112 (2021), Abstract
- ROS/JNK/C-Jun Pathway is Involved in Chaetocin Induced Colorectal Cancer Cells Apoptosis and Macrophage Phagocytosis Enhancement: Wang, H., Wen, C., et al.; Front. Pharmacol. 12, 729367 (2021), Abstract
- Impact of myeloid RIPK1 gene deletion on atherogenesis in ApoE-deficient mice: Coornaert, I., Puylaert, P., et al.; Atherosclerosis 322, 51 (2021), Abstract
- Docosahexaenoic acid protection against palmitic acid‐induced lipotoxicity in NGF‐differentiated PC12 cells involves enhancement of autophagy and inhibition of apoptosis and necroptosis: M.L. Montero, et al.; J. Neurochem. 155, 559 (2020), Abstract — Full Text
- CSF1R signaling is a regulator of pathogenesis in progressive MS: N. Hagan, et al.; Cell Death Dis. 11, 904 (2020), Abstract — Full Text
- Inhibitory role of TRIP-Br1/XIAP in necroptosis under nutrient/serum starvation: Z. Sandag, et al.; Mol. Cells 43, 236 (2020), Abstract — Full Text
- The transcription elongation factor TCEA3 induces apoptosis in rhabdomyosarcoma: N. Kazim, et al.; Cell Death Dis. 11, 67 (2020), Abstract — Full Text
- Molecular bases for HOIPINs-mediated inhibition of LUBAC and innate immune responses: D. Oikawa, et al.; Commun. Biol. 3, 163 (2020), Abstract — Full Text
- Bacterial type III effector protein HopQ inhibits melanoma motility through autophagic degradation of vimentin: S.H. Park, et al.; Cell Death Dis. 11, 231 (2020), Abstract — Full Text
- A novel neurodegenerative spectrum disorder in patients with MLKL deficiency: S.L. Faergeman, et al.; Cell Death Dis. 11, 303 (2020), Abstract — Full Text
- Autophagic death of neural stem cells mediates chronic stress-induced decline of adult hippocampal neurogenesis and cognitive deficits: Jung, S., Choe, S., et al.; Autophagy 16, 512 (2020), Abstract
- Autophosphorylation at serine 166 regulates RIP kinase 1-mediated cell death and inflammation: Laurien, L., Nagata, M., et al.; Nat. Commun. 11, 1747 (2020), Reactant(s): Mouse, Abstract
- CASP9 (caspase 9) is essential for autophagosome maturation through regulation of mitochondrial homeostasis: An, H. K., Chung, K. M., et al.; Autophagy 16, 1598 (2020), Abstract
- Locking mixed-lineage kinase domain-like protein in its auto-inhibited state prevents necroptosis: Rübbelke, M., Fiegen, D., et al.; PNAS 117, 33272 (2020), Abstract
- Preclinical antitumor efficacy of senescence-inducing chemotherapy combined with a nanoSenolytic: Galiana, I., Lozano-Torres, B., et al.; J. Control Release 323, 624 (2020), Abstract
- Loss of Microglial Parkin Inhibits Necroptosis and Contributes to Neuroinflammation: P.E.A. Dionisio, et al.; Mol. Neurobiol. 56, 2990 (2019), Abstract
- Advanced age protects resistance arteries of mouse skeletal muscle from oxidative stress through attenuating apoptosis induced by hydrogen peroxide: C.E. Norton, et al.; J. Physiol. 597, 3801 (2019), Abstract
- Gallic Acid Triggers Iron-Dependent Cell Death with Apoptotic, Ferroptotic, and Necroptotic Features: H.M. Tang & P.C.K. Cheung; Toxins 11, 492 (2019), Reactant(s): Human, Abstract
- Kinase-Based Screening of Marine Natural Extracts Leads to the Identification of a Cytotoxic High Molecular Weight Metabolite from the Mediterranean Sponge Crambe tailliezi: T.N. Nguyen, et al.; Mar. Drugs 17, 569 (2019), Abstract — Full Text
- Epigenetic Regulation of RIP3 Suppresses Necroptosis and Increases Resistance to Chemotherapy in NonSmall Cell Lung Cancer: Q. Wang, et al.; Transl. Oncol. 13, 372 (2019), Abstract — Full Text
- Chemical proteasome inhibition as a novel animal model of inner retinal degeneration in rats: M. Kageyama, et al.; PLoS One 14, e0217945 (2019), Abstract — Full Text
- Targeting the lysosome by an aminomethylated Riccardin D triggers DNA damage through cathepsin B‐mediated degradation of BRCA1: Y. Wang, et al.; J. Cell. Mol. Med. 23, 1798 (2019), Abstract — Full Text
- CIB1 depletion with docetaxel or TRAIL enhances triple-negative breast cancer cell death: A. H. Chung, et al.; Cancer Cell Int. 19, 26 (2019), Application(s): used w/ human triple-negative breast cancer cell lines (MDA-MB-436), MDA-MB-468, and MDA-MB-231 + huamn normal breast epithelial cell line (mE16C), Abstract — Full Text
- A luciferase-based assay identifies niclosamide derivatives antagonizing Mcl-1 through post-translational down-regulation: Liu, Q., Atkinson, J. M., et al.; bioRxiv , (2019)
- A NIK-SIX signalling axis controls inflammation by targeted silencing of non-canonical NF-κB: Liu, Z., Mar, K. B., et al.; Nature 568, 249 (2019), Abstract
- p75NTR and DR6 Regulate Distinct Phases of Axon Degeneration Demarcated by Spheroid Rupture: Yong, Y., Gamage, K. K., et al.; J. Neurosci. 39, 9503 (2019), Abstract
- Heme oxygenase-1 mediates BAY 11-7085 induced ferroptosis: L.C. Chang, et al.; Cancer Lett. 416, 124 (2018), Abstract
- Gasdermin D plays a vital role in the generation of neutrophil extracellular traps: G. Sollberger, et al.; Sci. Immunol. 3, eaar6689 (2018), Abstract
- To Be Or Not to Be: the Smoker’s Paradox” – An in-Vitro Study”””: F. Wittmann, et al.; Cell. Physiol. Biochem. 48, 1638 (2018), Abstract — Full Text
- The thiosemicarbazone Me2NNMe2 induces paraptosis by disrupting the ER thiol redox homeostasis based on protein disulfide isomerase inhibition: S. Hager, et al.; Cell Death Dis. 9, 1052 (2018), Abstract — Full Text
- Species-independent contribution of ZBP1/DAI/DLM-1-triggered necroptosis in host defense against HSV1: H. Guo, et al.; Cell Death Dis. 9, 816 (2018), Abstract — Full Text
- Proteasome inhibition blocks necroptosis by attenuating death complex aggregation: M. Ali, et al.; Cell Death Dis. 9, 346 (2018), Abstract — Full Text
- The sirtuin 1/2 inhibitor tenovin-1 induces a nonlinear apoptosis-inducing factor-dependent cell death in a p53 null Ewing’s sarcoma cell line: C. Marx, et al.; Invest. New Drugs 36, 396 (2018), Abstract
- Cylindromatosis mediates neuronal cell death in vitro and in vivo: G.K. Ganjam, et al.; Cell Death Differ. 25, 1394 (2018), Abstract — Full Text
- Autophagy Induced by Naftopidil Inhibits Apoptosis of Human Gastric Cancer Cells: A. Nakamura, et al.; Anticancer Res. 38, 803 (2018), Abstract
- PARP-1 inhibitors sensitize HNSCC cells to APR-246 by inactivation of thioredoxin reductase 1 (TrxR1) and promotion of ROS accumulation: Z.X. Yin, et al.; Oncotarget 9, 1885 (2018), Abstract — Full Text
- Intracellular Desmoglein-2 cleavage sensitizes epithelial cells to apoptosis in response to pro-inflammatory cytokines: M. Yulis, et al.; Cell Death Dis. 9, 389 (2018), Abstract — Full Text
- Endogenous hepcidin synthesis protects the distal nephron against hemin and hemoglobin mediated necroptosis: Van Swelm, R. P. L., Vos, M., et al.; Cell Death Dis. 9, 550 (2018), Reactant(s): Mouse, Abstract
- Expansion of DUB functionality generated by alternative isoforms – USP35, a case study: Leznicki, P., Natarajan, J., et al.; J. Cell Sci. 131, (2018), Abstract
- Lateral line placodes of aquatic vertebrates are evolutionarily conserved in mammals: Washausen, S., Knabe, W., et al.; Biol. Open 7, (2018), Abstract
- LT-IIc, A Bacterial Type II Heat-Labile Enterotoxin, Induces Specific Lethality in Triple Negative Breast Cancer Cells by Modulation of Autophagy and Induction of Apoptosis and Necroptosis: Masso-Welch, P., Girald Berlingeri, S., et al.; Int. J. Mol. Sci. 20, (2018), Abstract
- Mechanical cues control mutant p53 stability through a mevalonate-RhoA axis: Ingallina, E., Sorrentino, G., et al.; Nat. Cell Biol. 20, 28 (2018), Abstract
- Flow control effect of necrostatin-1 on cell death of the NRK-52E renal tubular epithelial cell line: J. Luo, et al.; Mol. Med. Rep. 16, 57 (2017), Reactant(s): Rat, Abstract — Full Text
- PEPD is a pivotal regulator of p53 tumor suppressor: L. Yang, et al.; Nat. Commun. 8, 2052 (2017), Abstract — Full Text
- Fangchinoline Induces Apoptosis, Autophagy and Energetic Impairment in Bladder Cancer: B. Fan, et al.; Cell. Physiol. Biochem. 43, 1003 (2017), Abstract — Full Text
- TRAIL-induces apoptosis but not necroptosis in colorectal and pancreatic cancer cells preferentially via the TRAIL-R2/DR5 receptor: Z. Nahacka, et al.; Biochim. Biophys. Acta 1865, 522 (2017), Application(s): Induction of TRAIL-mediated necroptosis on HT-29 cancer cells, Abstract
- Innovative substance 2250 as a highly promising anti-neoplastic agent in malignant pancreatic carcinoma – in vitro and in vivo: M. Buchholz, et al.; BMC Cancer 17, 216 (2017), Abstract — Full Text
- Strong antitumor synergy between DNA crosslinking and HSP90 inhibition causes massive premitotic DNA fragmentation in ovarian cancer cells: D. Kramer, et al.; Cell Death Differ. 24, 300 (2017), Abstract
- A trans-platinum(II) complex induces apoptosis in cancer stem cells of breast cancer: N. Aztopal, et al.; Bioorg. Med. Chem. 25, 269 (2017), Application(s): Cell treatment for the ATP viability assay, Abstract
- Deregulation of KSHV latency conformation by ER-stress and caspase-dependent RAD21-cleavage: De Leo, A., Chen, H. S., et al.; PLoS Pathog. 13, e1006596 (2017), Abstract
- Mitochondrial complex I inhibition triggers a mitophagy-dependent ROS increase leading to necroptosis and ferroptosis in melanoma cells: Basit, F., van Oppen, L. M., et al.; Cell Death Dis. 8, e2716 (2017), Abstract
- Necroptosis is a key mediator of enterocytes loss in intestinal ischaemia/reperfusion injury: Wen, S., Ling, Y., et al.; J. Cell. Mol. Med. 21, 432 (2017), Abstract
- PEBP1 Wardens Ferroptosis by Enabling Lipoxygenase Generation of Lipid Death Signals: Wenzel, S. E., Tyurina, Y. Y., et al.; Cell 171, 628 (2017), Abstract
- Phosphorylation of p62 by AMP-activated protein kinase mediates autophagic cell death in adult hippocampal neural stem cells: Ha, S., Jeong, S. H., et al.; J. Biol. Chem. 292, 13795 (2017), Abstract
- Rapid generation of hydrogen peroxide contributes to the complex cell death induction by the angucycline antibiotic landomycin E: Panchuk, R. R., Lehka, L. V., et al.; Free Radic. Biol. Med. 106, 134 (2017), Abstract
- Reciprocal amplification of caspase-3 activity by nuclear export of a putative human RNA-modifying protein, PUS10 during TRAIL-induced apoptosis: Jana, S., Hsieh, A. C., et al.; Cell Death Dis. 8, e3093 (2017), Abstract
- Very Long Chain Fatty Acids Are Functionally Involved in Necroptosis: Parisi, L. R., Li, N., et al.; Cell Chem. Biol. 24, 1445 (2017), Abstract
- The double benefit of Spalax p53: surviving underground hypoxia while defying lung cancer cells in vitro via autophagy and caspase-dependent cell death: M. Ellis, et al.; Oncotarget 7, 63242 (2016), Reactant(s): Human, Abstract — Full Text
- Caspase-3 Deletion Promotes Necrosis in Atherosclerotic Plaques of ApoE Knockout Mice: M.O. Grootaert, et al.; Oxid. Med. Cell Longev. 2016, 3087 (2016), Application(s): Cell culture, mouse bone marrow-derived macrophages, Abstract — Full Text
- Ritonavir Interacts With Belinostat to Cause Endoplasmic Reticulum Stress and Histone Acetylation in Renal Cancer Cells: M. Isono, et al.; Oncol. Res. 24, 327 (2016), Application(s): Flow cytometry, Human renal cancer cells, Abstract — Full Text
- The de-ubiquitylating enzyme DUBA is essential for spermatogenesis in Drosophila: L. Koerver, et al.; Cell Death Differ. 23, 2019 (2016), Application(s): DEVDase assay from testes lysate, Abstract
- Ammonium accumulation is a primary effect of 2-methylcitrate exposure in an in vitro model for brain damage in methylmalonic aciduria: H.P. Cudré-Cung, et al.; Mol. Genet. Metab. 119, 57 (2016), Application(s): Cell culture, blocking apoptosis, Abstract
- Organ specific alteration in caspase expression and STK3 proteolysis during the aging process: M. Lessard-Beaudoin, et al.; Neurobiol. Aging 47, 50 (2016), Application(s): Western blot analysis, Abstract — Full Text
- Increased apoptosis and browning of TAK1-deficient adipocytes protects against obesity: A. Sassmann-Schweda, et al.; JCI Insight 1, e81175 (2016), Application(s): Blocked increased caspase-3 activity, Abstract — Full Text
- Dasatinib promotes paclitaxel-induced necroptosis in lung adenocarcinoma with phosphorylated caspase-8 by c-Src: Y. Diao, et al.; Cancer Lett. 379, 12 (2016), Application(s): Cell culture, Abstract
- PKC activation sensitizes basal-like breast cancer cell lines to Smac mimetics: L. Cornmark, et al.; Cell Death Discov. 2, 16002 (2016), Application(s): Cell culture, Abstract — Full Text
- MASTL(Greatwall) regulates DNA damage responses by coordinating mitotic entry after checkpoint recovery and APC/C activation: P.Y. Wong, et al.; Sci. Rep. 6, 22230 (2016), Application(s): Cell culture, Abstract — Full Text
- The cellular apoptosis susceptibility protein (CAS) promotes TRAIL-induced apoptosis and cell proliferation: P. Monian & X. Jiang; J. Biol. Chem. 291, 2379 (2016), Application(s): Western blot, Abstract — Full Text
- Mechanism of neem limonoids-induced cell death in cancer: Role of oxidative phosphorylation: N. Yadav, et al.; Free Radic. Biol. Med. 90, 261 (2016), Application(s): Quantification of apoptosis and caspase activity measurement, Abstract
- Pyrrocidine A, a metabolite of endophytic fungi, has a potent apoptosis-inducing activity against HL60 cells through caspase activation via the Michael addition: S. Uesugi, et al.; J. Antibiot. (Tokyo) 69, 113 (2016), Application(s): DNA gel electrophoresis, Abstract — Full Text
- Calcineurin inhibitors cyclosporin A and tacrolimus protect against podocyte injury induced by puromycin aminonucleoside in rodent models: Shen, X., Jiang, H., et al.; Sci. Rep. 6, 32087 (2016), Abstract
- Modulation of P2X4/P2X7/Pannexin-1 sensitivity to extracellular ATP via Ivermectin induces a non-apoptotic and inflammatory form of cancer cell death: D. Draganov, et al.; Sci. Rep. 5, 16222 (2015), Abstract — Full Text
- The NLRP3 Inflammasome Is a Pathogen Sensor for Invasive Entamoeba histolytica via Activation of α5β1 Integrin at the Macrophage-Amebae Intercellular Junction: L. Mortimer, et al.; PLoS Pathog. 11, e1004887 (2015), Abstract — Full Text
- Characterization of Morphological and Cellular Events Underlying Oral Regeneration in the Sea Anemone, Nematostella vectensis: A.R. Amiel, et al.; Int. J. Mol. Sci. 16, 289449 (2015), Application(s): Blocked transcription, Abstract — Full Text
- Fibril growth and seeding capacity play key roles in α-synuclein-mediated apoptotic cell death: A.L. Mahul-Mellier, et al.; Cell Death Differ. 22, 2107 (2015), Application(s): Cell Culture, Abstract
- Guggulsterone inhibits human cholangiocarcinoma Sk-ChA-1 and Mz-ChA-1 cell growth by inducing caspase-dependent apoptosis and downregulation of survivin and Bcl-2 expression: F. Zhong, et al.; Oncol. Lett. 10, 1416 (2015), Application(s): Cell Culture, Abstract — Full Text
- The Tpl2 kinase regulates the COX-2/prostaglandin E2 axis in adipocytes in inflammatory conditions: F. Berthou, et al.; Mol. Endocrinol. 29, 1025 (2015), Application(s): Cell Culture, Abstract — Full Text
- USP30 deubiquitylates mitochondrial Parkin substrates and restricts apoptotic cell death: J.R. Liang, et al.; EMBO Rep. 16, 618 (2015), Application(s): Cell Culture, Abstract — Full Text
- The Wnt target Peter Pan defines a novel p53-independent nucleolar stress response pathway: A.S. Pfister, et al.; J. Biol. Chem. 290, 10905 (2015), Application(s): Cell Culture, Abstract — Full Text
- Cytochrome c oxidase deficiency accelerates mitochondrial apoptosis by activating ceramide synthase 6: S. Schüll, et al.; Cell Death Dis. 6, e1691 (2015), Application(s): Cell Culture, Abstract — Full Text
- Cellular responses to a prolonged delay in mitosis are determined by a DNA damage response controlled by Bcl-2 family proteins: D.J. Colin, et al.; Open Biol. 5, 140156 (2015), Application(s): Cell Culture, Abstract — Full Text
- Bile acid-induced necrosis in primary human hepatocytes and in patients with obstructive cholestasis: B. L. Woolbright, et al.; Toxicol. Appl. Pharmacol. 283, 168 (2015), Application(s): Cell Culture, Abstract
- Differential roles of RIPK1 and RIPK3 in TNF-induced necroptosis and chemotherapeutic agent-induced cell death: K. Moriwaki, et al.; Cell Death Dis. 6, e1636 (2015), Application(s): Cell Culture, Abstract — Full Text
- Involvement of JNK and Caspase Activation in Hoiamide A-Induced Neurotoxicity in Neocortical Neurons: Z. Cao, et al.; Mar. Drugs 13, 903 (2015), Application(s): Cell Culture, Abstract — Full Text
- Receptor-interacting protein 1 increases chemoresistance by maintaining inhibitor of apoptosis protein levels and reducing reactive oxygen species through a microRNA-146a-mediated catalase pathway: Wang, Q., Chen, W., et al.; J. Biol. Chem. 289, 5654 (2014), Abstract
- Tetrandrine blocks autophagic flux and induces apoptosis via energetic impairment in cancer cells: Qiu, W., Su, M., et al.; Cell Death Dis. 5, e1123 (2014), Abstract
- TLR4 Activation Under Lipotoxic Conditions Leads to Synergistic Macrophage Cell Death Through a TRIF-Dependent Pathway: J. D. Schilling et al.; J. Immunol. 190, 1285 (2013), Application(s): Inhibition of caspase in macrophages, Abstract — Full Text
- Internalization and induction of antioxidant messages by microvesicles contribute to the antiapoptotic effects on human endothelial cells: R. Soleti, et al.; Free Radic. Biol. Med. 53, 2159 (2012), Application(s): HUVEC cell culture, Abstract
- Inhibition of poly(ADP-ribose) polymerase (PARP) induces apoptosis in lung cancer cell lines: N.N. Gangopadhyay, et al.; Cancer Invest. 29, 608 (2011), Abstract
- Cytotoxicity of troglitazone through PPARγ-independent pathway and p38 MAPK pathway in renal cell carcinoma: M. Fujita, et al.; Cancer Lett. 312, 219 (2011), Abstract
- Type of cell death induced by various metal cations in cultured human gingival fibroblasts: R.G. Contreras, et al.; In Vivo 24, 513 (2010), Abstract
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Annexin V-FITC apoptosis detection kit
ALX-850-020
Easy, quick assay to distinguish apoptotic cells vs necrotic cells
| Application | Flow Cytometry, Fluorescence microscopy, Fluorescent detection |
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| Application | Flow Cytometry, Fluorescence microscopy |
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Datasheet, Manuals, SDS & CofA
Manuals And Inserts
Specific Protocol
Prepare a 10mM stock solution of the caspase inhibitor in high quality DMSO. Add 2µl of this stock solution to 1ml of culture medium containing cells to give 20µM final concentration. Effective final concentrations are estimated to be 5-20µM. If a particular caspase inhibitor is applied at higher concentrations specificity for an individual caspase or even for the caspase family (> 100µM) might be compromised. DMSO concentration above 0.2% may cause cellular toxicity thus masking the effect of the caspase inhibitor. For in vivo experiments extending for 12 to 48 hours, fresh inhibitor may have to be added (injected) due to inactivation by reaction with cysteine protease.
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