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VeriKine Mouse Interferon Alpha ELISA Kit

PBL-42120

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Detects global amount of mouse interferon alpha in tissue culture media as low as 12.5 pg/ml

Detects all 14 mouse IFN Alpha subtypes in tissue culture media (TCM)
Consistent performance and reproducible results with < 10% CVs

The VeriKine™ Mouse Interferon Alpha ELISA Kit has been developed to quantitate levels of IFN-alpha in tissue culture media using a sandwich immunoassay.

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Typical Mouse IFN Alpha standard curve from 12.5/ml to 400 pg/ml

Product Details

Alternative Name	IFN-α, Interferon-α
Application	ELISA
Application Notes	Quantitative determination of Mu-IFN Alpha in tissue culture media
Assay Time	Incubation time: 27 hours, 15 minutes
Contents	Pre-coated microtiter plate(s), Plate Sealers, Wash Solution Concentrate, Mouse Interferon Alpha (IFN-α) Standard (10,000 pg/ml), Sample Buffer, Concentrate Diluent, Antibody Concentrate, HRP Conjugate Concentrate, Assay Diluent, TMB Substrate Solution, Stop Solution
Crossreactivity	No-cross reactivity with rat IFN-alpha; mouse IFN-beta or gamma; or human IFN-alpha.
Sensitivity	12.5 – 400 pg/ml
Species Reactivity	Mouse
Specificity	Detects all 14 mouse IFN-alpha sub-types in tissue culture media.

Handling & Storage

Long Term Storage	+4°C
Shipping	Blue Ice

Regulatory Status	RUO – Research Use Only

Intranasal neomycin evokes broad-spectrum antiviral immunity in the upper respiratory tract: T. Mao, et al.; PNAS 121, e2319566121 (2024), Abstract
Gadd45b is critical for regulation of type I interferon signaling by facilitating G3BP-mediated stress granule formation: W.A.G. Chathuranga, et al.; Cell Rep. 42, 113358 (2023), Abstract
Levels of Influenza A Virus Defective Viral Genomes Determine Pathogenesis in the BALB/c Mouse Model: R. Penn, et al.; J. Virol. 96, e0117822 (2022), Abstract
IRF3-binding INcRNA-ISIR strengthens interferon production in viral infection and autoinflammation: J. Xu, et al.; Cell Rep. 37, 109926 (2021), Abstract
Myeloid neddylation targets IRF7 and promotes host innate immunity against RNA viruses: M. Zhao, et al.; PLoS Pathog. 17, e1009901 (2021), Abstract

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PBL-42400

Quantifies mouse interferon beta in tissue culture or serum to as low as 15.6 pg/ml

5 citations

Application	ELISA
Assay Time	Incubation time: 3 hours 15 minutes
Sensitivity	15.6 – 1000 pg/ml
Species Reactivity	Mouse

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VeriKine Mouse Interferon Alpha ELISA Kit

PBL-42120

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