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VeriKine Human Interferon Alpha Multi-Subtype Serum ELISA Kit

PBL-41110

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Human IFN-Alpha ELISA kit for measuring global human interferon alpha amount in serum, plasma, and tissue culture media (TCM)
Greater than 90% spike recovery in normal human serum
ELISA assay detects 11 of 12 major human IFN-alpha subtypes
Less than 10% inter and intra-assay CV

The VeriKine™ Human Interferon Alpha Multi-Subtype Serum ELISA kit is designed to provide accurate detection of human IFN-alpha in serum and plasma samples.

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Representative IFN-Alpha standard curve from 12.5 pg/ml to 1,000 pg/ml in standard diluent

Spike-Recovery of IFN-Alpha in different matrices

Product Details

Alternative Name	IFN-α, Interferon-α
Application	ELISA
Application Notes	Quantitative determination of Hu-IFN alpha A, 2, A/D, B2, C, D, G, H, I, J1, K, 1, 4b and WA in tissue culture media
Assay Time	Incubation time: 3 hours 15 minutes
Contents	Pre-coated microtiter plate(s), Plate Sealers, Wash Solution Concentrate, Human Interferon Alpha Standard (10,000 pg/ml), Sample Diluent, Antibody Concentrate, HRP Conjugate Concentrate, Concentrate Diluent, TMB Substrate, Stop Solution, Dilution Buffer
Crossreactivity	No cross reactivity with human interferon beta, gamma, omega; mouse or rat interferon alpha, beta, gamma; or bovine interferon tau.
Sensitivity	12.5 – 1000 pg/ml
Species Reactivity	Human

Handling & Storage

Long Term Storage	+4°C
Shipping	Blue Ice

Regulatory Status	RUO – Research Use Only

Gadd45b is critical for regulation of type I interferon signaling by facilitating G3BP-mediated stress granule formation: W.A.G. Chathuranga, et al.; Cell Rep. 42, 113358 (2023), Abstract
Evaluation of the interaction between tumor growth factor-b and interferon type I pathways in patients with COVID-19: factoring on ages 1 to 90 years: M. Abbasifard, et al.; BMC Infect. Dis. 23, 248 (2023), Abstract
Structural analysis reveals TLR7 dynamics underlying antagonism: S. Tojo, et al.; Nat. Commun. 11, 5204 (2020), Abstract
STAG2 deficiency induces interferon responses via cGAS-STING pathway and restricts virus infection: S. Ding, et al.; Nat. Commun. 9, 1485 (2018), Abstract

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VeriKine Human Interferon Alpha Multi-Subtype Serum ELISA Kit

PBL-41110

Product Details

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