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VeriKine-HS Mouse Interferon Beta Serum ELISA Kit

PBL-42410

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The world’s most sensitive Mouse IFN Beta ELISA

Mouse IFN-Beta ELISA with 0.94 pg/ml LLOQ for accurate quantitation of mouse interferon beta protein
Compatible with serum, plasma, and tissue cultre media (TCM)
Provides reproducible results with inter- & intra-assay CVs of < 10%
Total assay time of ~2 hours

PBL’s 96-well high sensitivity ELISA kit will equip you with the most sensitive and consistent tool for accurately measuring Mouse IFN-β in serum and plasma matrices. This kit quantitates Mouse Interferon Beta (IFN-β) in sera, plasma and tissue culture media by sandwich enyzme linked immunosorbent assay (ELISA). Interferon binds to plates coated with antibody and detection is accomplished using a detection antibody followed by streptavidin conjugated to horseradish peroxidase (HRP). This ELISA kit utilizes Tetramethyl-benzidine (TMB) as the substrate. Purified, recombinant mouse IFN-β expressed in mammalian cells is provided as the standard. The sensitivity provided by this kit, to less than 1 pg/ml in serum and plasma, will propel your mouse studies and help uncover insights into the pathology of diseases.

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Typical standard curve in a variety of matrices for PBL’s High-Sensitivity Mouse IFN-Beta ELISA (42410) and another supplier’s ELISA in assay buffer

Comparison of mammalian Cell- and E.coli-expressed Mouse IFN-Beta detection using PBL’s 42410 ELISA kit

Intra- and Inter-assay CVs and average percent recoveries using PBL’s high sensitivity Mouse IFN-Beta ELISA (42410)

Product Details

Alternative Name	IFN-β, Interferon-β
Application	ELISA
Application Notes	Quantitative determination of Mu-IFN Beta in serum, plasma and tissue culture media
Assay Time	Incubation time: 1 hour, 50 minutes
Contents	Pre-coated microtiter plate(s), Plate Sealers, Wash Solution Concentrate, Mouse Interferon Beta Standard (10,000 pg/ml), Sample Diluent, Serum Buffer, Antibody Concentrate, Antibody Diluent, HRP Conjugate Concentrate, HRP Diluent, TMB Substrate, Stop Solution
Crossreactivity	No cross-reactivity against mouse: IFN-α A, IFN-α 4. IFN-α 11. IFN-α 13, IFN-γ, IL-6, Limitin, TNF-α; human: IFN-α A, IFN-β, IFN-γ, IFN-ω; rat: IFN-α, IFN-β, IFN-γ; swine: IFN-α, IFN-β
Sensitivity	0.94 – 60 pg/ml
Species Reactivity	Mouse

Handling & Storage

Long Term Storage	+4°C
Shipping	Blue Ice

Regulatory Status	RUO – Research Use Only

Nucleotide modifications enable rational design of TLR7-selective ligands by blocking RNase cleavage: A.-J. Tong, et al.; J. Exp. Med. 221, e20230341 (2024), Abstract
Pulsed Electric Fields Induces STING Palmitoylation and Polymerization Independently of Plasmid DNA Electrotransfer: A. Sales Conniff, et al.; Pharmaceutics 16, 363 (2024), Abstract
New insights into the responder/nonresponder divide in rectal cancer: Damage-induced Type I IFNs dictate treatment efficacy and can be targeted to enhance radiotherapy: T.P. Uccello, et al.; Cell Death Dis. 14, 470 (2023), Abstract
Synthetic enforcement of STING signaling in cancer cells appropriates the immune microenvironment for checkpoint inhibitor therapy: L. Vornholz, et al.; Sci. Adv. 9, eadd8564 (2023), Abstract
Tau activation of microglial cGAS-IFN reduces MEF2C-mediated cognitive resilience: J.C. Udeochu, et al.; Nat. Neurosci. 26, 737 (2023), Abstract

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VeriKine-HS Mouse Interferon Beta Serum ELISA Kit

PBL-42410

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