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VeriKine-HS Human Interferon Alpha All Subtype ELISA Kit

PBL-41115

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Human IFN Alpha ELISA quantitates low level human interferon alpha in normal & autoimmune sera, EDTA plasma, and TCM with LLOQ 1.95 pg/ml
Detects All 12 human IFN Alpha subtypes for accurate total IFN alpha measurement in samples
Reproducible inter- (<10%) and intra-assay (< 8%) CVs in all tested matrices provide reliable data

This high sensitivity Human IFN Alpha ELISA kit is designed to provide accurate low pg/ml measurement of ALL human interferon alpha subtypes in autoimmune sera, normal serum, EDTA plasma, and tissue culture media (TCM).

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Representative Human IFN-Alpha Subtype standard curves in Healthy Donor Serum (HDS)

Representative Lower Limits of Quantification (LLOQ) and Lower Limits of Detection (LLOD) in Healthy Donor Serum for each human IFN-Alpha Subtype

IFN-Alpha level in Healthy and Influenza patient serum samples measured by PBL 41115-1 ELISA

Product Details

Alternative Name	IFN-α, Interferon-α
Application	ELISA
Application Notes	Quantitative determination of Hu-IFN alpha A, 2, A/D, B2, C, D, G, H, I, J1, K, 1, 4b and WA in serum, plasma and tissue culture media
Assay Time	Incubation time: 22 hours, 30 minutes
Contents	Pre-coated microtiter plate(s), Plate Sealers, Wash Solution Concentrate, Human Interferon Alpha Standard (10,000 pg/ml), Sample Buffer, Standard Diluent, Antibody Concentrate, HRP Conjugate Concentrate, Antibody Diluent, HRP Diluent, TMB Substrate, Stop Solution
Crossreactivity	Cross-reacts with Cynomolgus/Rhesus IFN-α. No cross-reactivity against human IFN-β, IFN-γ, or IFN-ω; mouse or rat IFN-α, IFN-β or IFN-γ; or bovine IFN-τ.
Sensitivity	1.95-125 pg/ml
Species Reactivity	Human

Handling & Storage

Long Term Storage	+4°C
Shipping	Blue Ice

Regulatory Status	RUO – Research Use Only

In Vitro and In Vivo Crosstalk between Type I IFN and IL-8 Responses in SARS-CoV-2 Infection: M. Biava, et al.; Microorganisms 11, 2787 (2023), Abstract
Reduction of IFN-I Responses by Plasmacytoid Dendritic Cells in a Longitudinal Trans Men Cohort: B. Grünhagel, et al.; iScience 26, 108209 (2023), Abstract
Dominant CT Patterns and Immune Responses during the Early Infection Phases of Different SARS-CoV-2 Variants: K. Nagaoka, et al.; Viruses 15, 1304 (2023), Abstract
Interferon and interferon-induced cytokines as markers of impending clinical progression in ANA+ individuals without a systemic autoimmune rheumatic disease diagnosis: S.T. Kim, et al.; Arthritis Res. Ther. 25, 21 (2023), Abstract
Restoration of dendritic cell homeostasis and Type I/Type III interferon levels in convalescent COVID-19 individuals: A. Rajamanickam, et al.; BMC Immunol. 23, 51 (2022), Abstract

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VeriKine-HS Human Interferon Alpha All Subtype ELISA Kit

PBL-41115

Product Details

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