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VeriKine Cynomolgus/Rhesus Interferon Alpha Serum ELISA Kit

PBL-46100

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Quantifies non-human primate IFN alpha in serum, plasma, or tissue culture media to as low as 25 pg/ml

Quantifies Cynomolgus and Rhesus IFN-Alpha 2 in serum, plasma, and tissue culture media (TCM)
Reproducible results with tissue culture media inter-assay CV ≤ 9.2% and intra-assay CV ≤ 2.5% (serum ≤ 14.4% and ≤ 9.2%, respectively)
Recombinant Rhesus/Cyno IFN Alpha 2 provided as the ELISA standard

The Cynomolgus/Rhesus ELISA Kit has been developed to analyze the presence of non-human primate IFN-alpha in tissue culture media or serum samples by sandwich enzyme linked immunosorbent assay (ELISA).

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Spike-recovery of Cynomolgus/Rhesus IFN-Alpha in serum, plasma, TCM and 46100 kit’s sample buffer

Representative Cynomolgus IFN-Alpha standard curve from 25-1,600 pg/ml

Product Details

Alternative Name	IFN-α, Interferon-α
Application	ELISA
Application Notes	Quantitative determination of Cynomolgus/Rhesus Macaque IFN Alpha in tissue culture media, serum and plasma
Assay Time	Incubation time: 3 hours 15 minutes
Contents	Pre-coated microtiter plate, Plate sealers, Wash Solution Concentrate, Rhesus/Cyno Interferon Alpha 2 Standard (10,000 pg/ml), Standard Diluent, Sample Buffer, Antibody Concentrate, HRP Conjugate Concentrate, Concentrate Diluent, TMB Substrate, Stop Solution
Crossreactivity	Strong cross-reactivity against human IFN alpha2; No cross-reactivity with human IFN gamma; or mouse and rat IFN alpha.
Sensitivity	25 – 1600 pg/ml
Species Reactivity	Cynomolgus, Rhesus monkey

Handling & Storage

Long Term Storage	+4°C
Shipping	Blue Ice

Regulatory Status	RUO – Research Use Only

Immune Activation Profiles Elicited by Distinct, Repeated TLR Agnoist Infusions in Rhesus Macaques: H.A.D. King, et al.; J. Immunol. 211, 1643 (2023), Abstract
Single-dose VSV-based vaccine protects cynomolgus macaques from disease after Tai Forest virus infection: P. Fletcher, et al.; Emerg. Microbes Infect. 12, 2239950 (2023), Abstract
TLR7 agonist administration to SIV-infected macaques receiving early initiated cART does not induce plasma viremia: G.Q. Del Prete, et al.; JCI Insight 4, e127717 (2019), Abstract
Inhibition of p38MAPK in combination with ART reduces SIV-induced immune activation and provides additional protection from immune system deterioration: O. Chaudhary, et al.; PLoS Pathog. 14, e1007268 (2018), Abstract
Innate transcriptional effects by adjuvants on the magnitude, quality, and durability of HIV envelope responses in NHPs: J.R. Francica, et al.; Blood. Adv. 1, 2329 (2017), Abstract

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VeriKine Cynomolgus/Rhesus Interferon Alpha Serum ELISA Kit

PBL-46100

Product Details

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