VEGFR2 (human), (recombinant) (GST-tag)

KDR, FLK-1, Vascular endothelial growth factor receptor 2

Useful tool to study enzyme regulation and kinetics, phosphorylate target substrates, screen for inhibitors.

Liquid. In 40 mM TRIS, pH 8.0, containing 110 mM NaCl, 2.2 mM KCl, 0.02% Tween-10, 20% glycerol, and 3 mM DTT.

AF035121

~87 kDa

≥90% (SDS-PAGE)

Produced in Sf9 insect cells with N-terminal GST tage. Human VEGFR2 (aa 805-1356). Produced in a baculovirus expression system.

≥400 pmol/min/µg. Assay was done in Kinase buffer containing 0.2 mM DTT using Poly- (Glu4:Tyr)-biotin substrate (0.2 mg/ml) and 20 μM ATP. Reaction was done at 30°C for 40 min. Amount of ATP transferred was calculated using Kinase-Glo reagent (Promega).

P35968