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This antibody detects Voltage-Dependent Anion Channel 2 (VDAC2), which is involved in mitochondrial permeability and apoptosis regulation.
VDAC2 is a mitochondrial outer membrane protein involved in metabolic regulation and apoptosis. It interacts with apoptotic proteins like Bcl-2 and Bax, making it an important target in cancer and neurodegenerative diseases.
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Western blot analysis of various lysates using VDAC2 rabbit polyclonal antibody (ENZ-ABS1072) at 1:500 dilution. Secondaryu Antibody: Goat Anti-Rabbit IgG (H+L)/HRP at 1:10’000 dilution. Lane A: HeLa Whole Cell Lysate, Lane B: HepG2 Whole Cell Lysate, Lane C: HCT116 Whole Cell Lysate, Lane D: U-251 MG Whole Cell Lysate. Lysates/proteins at 30 μg per lane.
Immunochemical staining of human VDAC2 in human kidney with VDAC2 rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.
Immunochemical staining of human VDAC2 in cynomolgus cerebellum with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.
Immunofluorescence staining of VDAC2 in U2OS cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit VDAC2 polyclonal antibody (dilution ratio 1:200) at 4°C overnight. Then cells were stained with the Alexa Fluor®488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to Cytoplasm.
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RUO – Research Use Only |
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