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VAMP2 polyclonal antibody

ADI-VAS-SV006

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Vesicle-associated membrane protein (VAMP), also known as synaptobrevin, is an 18 kDa integral membrane protein localized to the cytoplasmic surface of synaptic vesicles. Two VAMP homologs, VAMP1 and VAMP2, are differentially expressed in the nervous system. VAMP2 is found in neurons associated with autonomic, sensory and integrative functions, and in non-neuronal Langerhans islets and glomerular cells. VAMP is a component of a 20S complex implicated in vesicle docking and fusion, the formation of which involves the interaction of VAMP with syntaxin through an ATP-dependent reaction modulated by synaptotagmin, SNAP-25, alpha-SNAP, and NSF.

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VAMP2 polyclonal antibody Western blot — Western blot analysis of VAMP2, pAb: Lane 1: Molecular Weight Marker, Lane 2: Mouse Brain Tissue Extract (Prod. No.: ADI-LYT-MB100), Lane 3: Rat Brain Tissue Extract (Prod. No.: ADI-LYT-RB100)

Product Details

Alternative Name	Synaptobrevin, Vesicle-associated membrane protein 2
Application	ICC, IP, WB
Application Notes	Detects a band of ~18kDa by Western blot.
Formulation	Liquid. In PBS, pH 7.2, containing 50% glycerol and 0.09% sodium azide.
GenBank ID	M24105
Host	Rabbit
Immunogen	Synthetic peptide corresponding to a portion of rat VAMP2. The sequence is completely conserved in mouse, human, bovine, and Xenopus.
Purity Detail	Protein A affinity purified.
Recommendation Dilutions/Conditions	Western Blot (1:1,000, ECL)Suggested dilutions/conditions may not be available for all applications.Optimal conditions must be determined individually for each application.
Source	Purified from rabbit serum.
Species Reactivity	Bovine, Guinea pig, Human, Mouse, Rabbit, Rat
UniProt ID	P63045
Worry-free Guarantee	This antibody is covered by our Worry-Free Guarantee.

Handling & Storage

Long Term Storage	-20°C
Shipping	Blue Ice

Regulatory Status	RUO – Research Use Only

ELKS/Voltage-Dependent Ca2+ Channel-β Subunit Module Regulates Polarized Ca2+ Influx in Pancreatic β Cells: M. Ohara-Imaizumi, et al.; Cell Rep. 26, 1213 (2019), Abstract
Pancreatic acinar cells express vesicle-associated membrane protein 2-and 8-specific populations of zymogen granules with distinct and overlapping roles in secretion: G. Groblewski, et al.; J. Biol. Chem. 282, 9635 (2007), Application(s): WB, IP using rat tissue, Abstract
Cytoplasmic dynein participates in apically targeted stimulated secretory traffic in primary rabbit lacrimal acinar epithelial cells: S.F. Hamm-Alvarez, et al.; J. Cell. Sci. 116, 2051 (2003), Application(s): WB using rabbit samples, Abstract
Ca2+-dependent formation of a dynamin-synaptophysin complex: potential role in synaptic vesicle endocytosis: E.B. Ziff, et al.; J. Biol. Chem. 277, 9010 (2002), Application(s): IP, WB using rat samples, Abstract
Transduction of MIN6 beta cells with TAT-syntaxin SNARE motif inhibits insulin exocytosis in biphasic insulin release in a distinct mechanism analyzed by evanescent wave microscopy: S. Nagamatsu, et al.; J. Biol. Chem. 277, 50805 (2002), Application(s): IP using mouse samples, Abstract
Subcellular distribution of 3 functional platelet SNARE proteins: human cellubrevin, SNAP-23, and syntaxin 2: D. Feng, et al.; Blood 99, 4006 (2002), Application(s): WB using bovine & human samples, Abstract
Perinuclear localization and insulin responsiveness of GLUT4 requires cytoskeletal integrity in 3T3-L1 adipocytes: M.P. Czech, et al.; J. Biol. Chem. 275, 38151 (2000), Application(s): WB using mouse samples, Abstract
Human granulocytic ehrlichiosis agent and Ehrlichia chaffeensis reside in different cytoplasmic compartments in HL-60 cells: Y. Rikihisa, et al.; Infect. Immun. 67, 1368 (1999), Application(s): ICC using human samples, Abstract