- Quantitate PGI2 – measuring the stable breakdown products 6-keto-PGF1α and 2,3-dinor-6keto-PGF1α in one simple assay
- Less stress – much easier than GC/MS with results from up to 39 samples in duplicate in under 3 hours
- Specific – low cross-reactivity with related eicosanoids
- Save with bulk kit package!
The Urinary Prostacyclin EIA kit is a colorimetric competitive enzyme immunoassay kit with results in < 3 hours. Absorbance is read at 405 nm. Quantitate PGI2 by measuring the stable metabolites in one simple assay.
Prostacyclin (PGI2) is involved in platelet aggregation, vasoconstriction, and reproductive functions. PGI2 has a half life of 60 minutes in plasma but only 2 to 3 minutes in buffer. The production of PGI2 is typically monitored by measurement of 6-keto-PGF1α and 2,3-dinor-6-keto-PGF1α. 6-keto-PGF1α is produced by the non-enzymatic hydration of PGI2, and is further metabolized in urine to the 2,3-dinor derivative. A number of pharmaceuticals alter and/or inhibit the synthesis of PGI2. Methods to measure PGI2 in urine typically involve HPLC, gas chromatography/mass spectrometry, or radioimmunoassay and enzyme immunoassay for 6-keto-PGF1α. The conversion of PGI2 into 6-keto-PGF1α and 2,3-dinor-6-keto-PGF1α is shown below.
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Regulatory Status |
RUO – Research Use Only |
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