Urinary prostacyclin ELISA kit

• Quantitate PGI₂ – measuring the stable breakdown products 6-keto-PGF_1α and 2,3-dinor-6keto-PGF_1α in one simple assay
• Less stress – much easier than GC/MS with results from up to 39 samples in duplicate in under 3 hours
• Specific – low cross-reactivity with related eicosanoids

The Urinary Prostacyclin EIA kit is a colorimetric competitive enzyme immunoassay kit with results in < 3 hours. Absorbance is read at 405 nm. Quantitate PGI₂ by measuring the stable metabolites in one simple assay.

Prostacyclin (PGI2) is involved in platelet aggregation, vasoconstriction, and reproductive functions. PGI₂ has a half life of 60 minutes in plasma but only 2 to 3 minutes in buffer. The production of PGI₂ is typically monitored by measurement of 6-keto-PGF_1α and 2,3-dinor-6-keto-PGF_1α. 6-keto-PGF_1α is produced by the non-enzymatic hydration of PGI₂, and is further metabolized in urine to the 2,3-dinor derivative. A number of pharmaceuticals alter and/or inhibit the synthesis of PGI₂. Methods to measure PGI₂ in urine typically involve HPLC, gas chromatography/mass spectrometry, or radioimmunoassay and enzyme immunoassay for 6-keto-PGF_1α. The conversion of PGI₂ into 6-keto-PGF_1α and 2,3-dinor-6-keto-PGF_1α is shown below.