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Ubiquitin polyclonal antibody

ADI-SPA-200

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Ubiquitin (Ub) plays a very important role in regulated non-lysosomal ATP dependent protein degradation. The protein to be degraded is conjugated to Ub and the ubiquinated protein is then selectively degraded by the 26S complex, a multicatalytic cytosolic and nuclear protease. The Ub-proteasome proteolytic pathway, which is a complex process, is implicated to be of great importance for regulating numerous cellular processes.

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This antibody is covered by our Worry-Free Guarantee.

Ubiquitin polyclonal antibody Immunohistochemistry — Immunohistochemistry analysis of human brain, cortex, senile plaque tissue stained with Ubiquitin, pAb at 10μg/ml.

Ubiquitin polyclonal antibody Western blot — Western blot analysis: Ubiquitin protein probed with Ubiquitin pAb.

Product Details

Application	ICC, IHC (PS), WB
Application Notes	Detects a band of ~8kDa by Western blot.
Formulation	Liquid. In PBS, pH 7.2, containing 50% glycerol and 0.09% sodium azide.
Gene/Protein Identifier	NP_776558 (RefSeq)
Host	Rabbit
Immunogen	Native bovine Ubiquitin.
Purity Detail	Protein A affinity purified.
Recommendation Dilutions/Conditions	Western Blot (1:5,000, ECL)Suggested dilutions/conditions may not be available for all applications.Optimal conditions must be determined individually for each application.
Source	Purified from rabbit serum.
Species Reactivity	Bovine, Chicken, Dog, Drosophila, Fish, Guinea pig, Hamster, Human, Monkey, Mouse, Porcine, Rabbit, Rat, Sheep, Xenopus, Yeast
UniProt ID	P0CG53
Worry-free Guarantee	This antibody is covered by our Worry-Free Guarantee.

Handling & Storage

Handling	Avoid freeze/thaw cycles.
Long Term Storage	-20°C
Shipping	Blue Ice

Regulatory Status	RUO – Research Use Only

Impact of ATF6 deletion on the embryonic brain development: Nguyen, L. D., Nguyen, L. H., et al.; iScience 28, 112569 (2025), Abstract
An integrated proteomics approach identifies phosphorylation sites on viral and host proteins that regulate West Nile virus infection: Walter, Z., Li, M., et al.; Cell Rep. 44, 115728 (2025), Abstract
Biologically significant interaction of human herpesvirus 8 viral interferon regulatory factor 4 with ubiquitin-specific protease 7.: Yang, Z., Nicholas, J., et al.; J. Virol. 98, e0025524 (2024), Reactant(s): Human, Abstract
Atl (atlastin) regulates mTor signaling and autophagy in Drosophila muscle through alteration of the lysosomal network: Srivastav, S., van der Graaf, K., et al.; Autophagy 20, 131 (2024), Abstract
PNPLA3-I148M is a Neomorph that Interferes with Two Primary Hepatic Triglyceride Clearance Pathways: Sherman, D. J., Liu, L., et al.; bioRxiv , (2024)
USP41 Enhances Epithelial–Mesenchymal Transition of Breast Cancer Cells through Snail Stabilization: J.Y. Yoon, et al.; Int. J. Mol. Sci. 24, 1693 (2023), Abstract
Nuclear aging in polyglutamine-induced neurodegeneration: Pereira, D., Cunha-Santos, J., et al.; bioRxiv , (2023)
MLN4924 Inhibits Defective Ribosomal Product Antigen Presentation Independently of Direct NEDDylation of Protein Antigens: K. Vijayasimha, et al.; J. Immunol. 208, 2273 (2022), Abstract
Disrupting the ghrelin-growth hormone axis limits ghrelin’s orexigenic but not glucoregulatory actions: R.S. Eshaq, et al.; Microcirculation 28, e12717 (2021), Abstract
USP18 promotes tumor metastasis in esophageal squamous cell carcinomas via deubiquitinating ZEB1: C. Song, et al.; Exp. Cell Res. 409, 112884 (2021), Abstract

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Last modified: May 29, 2024

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Ubiquitin polyclonal antibody

ADI-SPA-200

Product Details

Handling & Storage

Datasheet, Manuals, SDS & CofA

Manuals And Inserts

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