Home Shop Ubiquitin monoclonal antibody (P4G7-H11)

Ubiquitin monoclonal antibody (P4G7-H11)

ADI-SPA-203

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Ubiquitin (Ub) plays a very important role in regulated non-lysosomal ATP dependent protein degradation. The protein to be degraded is conjugated to Ub and the ubiquinated protein is then selectively degraded by the 26S complex, a multicatalytic cytosolic and nuclear protease. The Ub-proteasome proteolytic pathway, which is a complex process, is implicated to be of great importance for regulating numerous cellular processes.

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This antibody is covered by our Worry-Free Guarantee.

Ubiquitin monoclonal antibody (P4G7-H11) Flow Cytometry — Flow cytometry analysis of human hepatoma QGY cells analyzed by flow cytometry using isotype control antibody (left) or Ubiquitin Monoclonal Antibody (P4G7-H11) (right) at 10µg/mL

Ubiquitin monoclonal antibody (P4G7-H11) Immunohistochemistry — Immunohistochemistry analysis of human brain, cortex tissue stained with Ubiquitin, mAb (P4G7-H11) at 5-10µg/ml.

Ubiquitin monoclonal antibody (P4G7-H11) Western blot — Western blot analysis of Ubiquitin: Lane 1: MW marker, Lane 2: Ubiquitin, Lane 3: HeLa, Lane 4: RK-13, Lane 5: 3T3, Lane 6: PC-12, Lane 7: Mouse Brain, Lane 8: Rat Brain, Lane 9: MDBK

ADI-SPA-203 Ubiquitin monoclonal antibody (P4G7-H11) Western Blot Rat 1 — Comparison of anti-ubiquitin antibodies.Heart and liver lysates (20 μg each) were investigated by Western blotting using five commercially available anti-ubiquitin antibodies (VU101, U5379, AP1228a, P4G7-H11, FK1). Arrow shows location of free unbound ubiquitin. Stain-free staining of total proteins loaded was used as the normalization control. H, heart; L, liver. BSA was used as the blocking reagent for the blot labeled FK1* while non-fat milk was used as the blocking reagent in all the other blots shown. All antibodies were used at a dilution of 1:1000 except for blots labeled U5379* and U5379^ which were used at dilutions of 1:100 and 1:2000 respectively.

Image collected and cropped by CiteAb under a CC-BY license from the following publication: Western Blotting Inaccuracies with Unverified Antibodies: Need for a Western Blotting Minimal Reporting Standard (WBMRS). PLoS One (2015)

Product Details

Application	Flow Cytometry, IHC (PS), WB
Application Notes	Detects a band of ~6.5kDa by Western blot.
Clone	P4G7-H11
Formulation	Liquid. In PBS containing 50% glycerol and 0.09% sodium azide.
Gene/Protein Identifier	NP_776558 (RefSeq)
Host	Mouse
Immunogen	Native bovine Ubiquitin.
Isotype	IgG1
Purity Detail	Protein G affinity purified.
Recommendation Dilutions/Conditions	Flow Cytometry (10µg/ml)Western Blot (1:1,000, colorimetric)Suggested dilutions/conditions may not be available for all applications.Optimal conditions must be determined individually for each application.
Source	Purified from ascites.
Species Reactivity	Bovine, Hamster, Human, Mouse, Rabbit, Rat
UniProt ID	P0CG53
Worry-free Guarantee	This antibody is covered by our Worry-Free Guarantee.

Handling & Storage

Handling	Avoid freeze/thaw cycles.
Long Term Storage	-20°C
Shipping	Blue Ice

Regulatory Status	RUO – Research Use Only

Transcription factor Nrf1 regulates proteotoxic stress-induced autophagy: Ward, M. A., Vangala, J. R., et al.; J. Cell Biol. 223, (2024), Application(s): WB, Abstract
Expression quantitative trait loci mapping identified PtrXB38 as a key hub gene in adventitious root development in Populus: T. Yao, et al.; New Phytol. , (2023), Abstract
Ubiquitin accumulation induced by the finger and palm sub-domains of NS5 modulates the replication of West Nile virus: S. Kobayashi, et al.; Virology 588, 109902 (2023), Abstract
Antibody targeting of E3 ubiquitin ligases for receptor degradation: H. Marei, et al.; Nature 610, 182 (2022), Abstract
Deubiquitinases USP20/33 promote the biogenesis of tail-anchored membrane proteins.: Mariappan, M., Culver, J. A., et al.; J. Cell Biol. 220, (2021), Application(s): WB, Abstract
Real-time tracking of complex ubiquitination cascades using a fluorescent confocal on-bead assay.: Koszela, J., Pham, N. T., et al.; BMC Biol. 16, 88 (2018), Application(s): WB / Reactant(s): E. coli, Abstract
Lifespan differences between queens and workers are not explained by rates of molecular damage: E.R. Lucas, et al.; Exp. Gerontol. 92, 1 (2017), Abstract
Stimulation of RNA Polymerase II ubiquitination and degradation by yeast mRNA 3′-end processing factors is a conserved DNA damage response in eukaryotes.: Moore, C., Kuehner, J. N., et al.; DNA Repair (Amst.) 57, 151 (2017), Reactant(s): Saccharomyces cerevisiae, Abstract
Transcriptional Induction of Metallothionein by Tris(pentafluorophenyl)stibane in Cultured Bovine Aortic Endothelial Cells: T. Fujie, et al.; Int. J. Mol. Sci. 17, 1381 (2016), Application(s): Western blot analysis of protein expression, Abstract — Full Text
Distinct types of translation termination generate substrates for ribosome-associated quality control: N. Shcherbik, et al.; Nucleic Acids Res. 44, 6840 (2016), Application(s): Cell analysis of the modification of distinct ribosomal species, Abstract — Full Text