Home Shop Ubiquitin monoclonal antibody (P4D1) (HRP conjugate)

Ubiquitin monoclonal antibody (P4D1) (HRP conjugate)

BML-PW0935

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Ubiquitin modification of cellular proteins has been shown to be involved with a wide range of biochemical processes including proteasomal degradation, signal transduction, DNA repair, endocytosis and autophagy.

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Product Details

Application	WB
Clone	P4D1
Formulation	Liquid. In PBS containing 0.01% sodium azide.
Host	Mouse
Immunogen	Denatured bovine ubiquitin.
Isotype	IgG1
Purity Detail	Ion exchange purified.
Recommendation Dilutions/Conditions	Western Blot (1:1,000)Suggested dilutions/conditions may not be available for all applications.Optimal conditions must be determined individually for each application.
Source	Purified from tissue culture supernatant. HRP conjugated chemically in vitro.
Species Reactivity	Species independent
Specificity	Recognizes mono- and poly-ubiquitin protein conjugates, free polyubiquitin chains and free ubiquitin.
Technical Info / Product Notes	P4D1 antibody was developed using a denatured glutaraldehyde cross-linked ubiquitin IgG complex as the immunogen. Monoclonal antibody was purified from tissue culture supernatant by ion exchange chromatography. The general ubiquitin reactivity of the monoclonal antibody has been demonstrated by Western blotting using synthetic ubiquitin chains, single isopeptide-linked polyubiquitin chains, and with endogenously ubiquitinylated species in cell lysates.
UniProt ID	P0CG53 (UBB), P0CH28 (UBC), P62992 (RPS27A), P63048 (UBA52)
Worry-free Guarantee	This antibody is covered by our Worry-Free Guarantee.

Handling & Storage

Handling	Avoid freeze/thaw cycles.
Short Term Storage	+4°C
Long Term Storage	+4°C
Shipping	Blue Ice

Regulatory Status	RUO – Research Use Only

Inhibition of USP1 enhances anticancer drugs-induced cancer cell death through downregulation of survivin and miR-216a-5p-mediated upregulation of DR5: S.M. Woo, et al.; Cell Death Dis. 13, 821 (2022), Abstract
Multiubiquitination of TRPV4 reduces channel activity independent of surface localization: Aisenberg, W. H., McCray, B. A., et al.; J. Biol. Chem. 298, 101826 (2022), Abstract
m6A modification of a 3′ UTR site reduces RME1 mRNA levels to promote meiosis.: Bartel, D. P., Lewis, C., et al.; Nat. Commun. 10, 3414 (2019), Application(s): WB, Abstract
Ubiquitination of ERMES components by the E3 ligase Rsp5 is involved in mitophagy.: Cohen, M. M., Belgareh-Touzé, N., et al.; Autophagy 13, 114 (2017), Application(s): WB / Reactant(s): Saccharomyces cerevisiae, Abstract
An ubiquitin-dependent balance between mitofusin turnover and fatty acids desaturation regulates mitochondrial fusion.: Erpapazoglou, Z., Weissman, A. M., et al.; Nat. Commun. 8, 15832 (2017), Application(s): WB / Reactant(s): Saccharomyces cerevisiae, Abstract
Activation of nuclear PTEN by inhibition of Notch signaling induces G2/M cell cycle arrest in gastric cancer: S.J. Kim, et al.; Oncogene 35, 251 (2016), Application(s): Western Blot, Abstract
FADD regulates NF-κB activation and promotes ubiquitination of cFLIPL to induce apoptosis: Ranjan, K., Pathak, C., et al.; Sci. Rep. 6, 22787 (2016), Abstract
Granzyme M targets topoisomerase II alpha to trigger cell cycle arrest and caspase-dependent apoptosis: de Poot, S. A., Lai, K. W., et al.; Cell Death Differ. 21, 416 (2014), Abstract
ZRANB3 is a structure-specific ATP-dependent endonuclease involved in replication stress response: Weston, R., Peeters, H., et al.; Genes Dev. 26, 1558 (2012), Abstract

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Ubiquitin monoclonal antibody (P4D1) (HRP conjugate)

BML-PW0935

Product Details

Handling & Storage

Datasheet, Manuals, SDS & CofA

Manuals And Inserts

Certificate of Analysis

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