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Ubiquitin-AMC

BML-SE211

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High purity industry standard substrate for deubiquitinylating enzymes (DUBs).

Ubiquitin-AMC is a fluorogenic substrate for a wide range of deubiquitinylating enzymes (DUBs), including ubiquitin C-terminal hydrolases (UCHs) and ubiquitin specific proteases (USPs). It is a particularly useful reagent for the study of deubiquitinylating activity where detection sensitivity or continuous monitoring of activity is essential. Ubiquitin-AMC has been shown to be a sensitive substrate for UCH-L3 (Prod. No. BML-UW9745) and for Isopeptidase-T (Prod. No. BML-UW9690), and is particularly useful for studying deubiquitinylating activity where detection sensitivity or continuous monitoring of activity is essential.

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Ubiquitin-AMC b — **Figure**: DUB Assay: 50nM UCLH1, His6-tagged (UW9740); 500nM Ubiquitin-AMC; 50mM HEPES pH7.8, 0.5mM EDTA, 1mM DTT.

Ubiquitin-AMC image — **Figure:** HPLC analysis. Comparison of Prod. No. BML-SE-211Prod. No. BML-SE-211) **(A)** with contaminated alternatively sourced material **(B)**.

Product Details

Application Notes	Can be used for the following applications: 1. Substrate for deubiquitinylating enzyme activity assays. 2. Identification/confirmation of enzyme deubiquitinylation activity. 3. Investigation of deconjugating enzyme substrate specificity in comparison with alternative UBL substrates.
Formulation	In DMSO
Purity	≥95% (HPLC)
Purity Detail	Please note: considerable efforts are taken to ensure that ubiquitin-AMC is homogeneous and not contaminated with ubiquitin and ubiquitin deletions, which are common in material obtained from other sources. The chromatogram below demonstrates the homogeneity of BML-SE211.
Source	Produced in E.coli.
UniProt ID	P0CG47 (UBB), P0CG48 (UBC), P62979 (RPS27A), P62987 (UBA52)

Handling & Storage

Use/Stability	As indicated on product label or CoA when stored as recommended.
Handling	Avoid freeze/thaw cycles.
Long Term Storage	-80°C
Shipping	Dry Ice

Regulatory Status	RUO – Research Use Only

Discovery of a Dual SENP1 and SENP2 Inhibitor: Brand, M., Bommeli, E. B., et al.; Int. J. Mol. Sci. 23, (2022), Abstract
Sem1/DSS1 accelerates ATP-dependent substrate unfolding by the proteasome through a conformation-dependent intercomplex contact: Reed, R. G., Jobin, G. W., et al.; bioRxiv , (2022)
Inhibition of the ubiquitin-proteasome system by a bioactivatable compound: Giovannucci, T. A., Salomons, F. A., et al.; bioRxiv , (2021)
Inhibition of the ubiquitin-proteasome system by an NQO1-activatable compound: Giovannucci, T. A., Salomons, F. A., et al.; Cell Death Dis. 12, 914 (2021), Abstract
Agouti Related Peptide Secreted Via Human Mesenchymal Stem Cells Upregulates Proteasome Activity in an Alzheimer’s Disease Model: N.K. Lee, et al.; Sci. Rep. 7, 39340 (2017), Abstract — Full Text
Evaluation of polyphenols from Broussonetia papyrifera as coronavirus protease inhibitors: J.Y. Park, et al.; J. Enzyme Inhib. Med. Chem. 32, 504 (2017), Application(s): SARS-CoV deubiquitination assay, Abstract — Full Text
Ubiquitin Carboxy-Terminal HydrolaseL3 Correlates with Human Sperm Count, Motility and Fertilization: M. Wang, et al.; PLoS One 11, e0165198 (2016), Application(s): Determination of UCH enzymatic activity of spermatozoa , Abstract — Full Text
Substrate profiling of deubiquitin hydrolases with a positional scanning library and mass spectrometry: D. E. Mason et al.; Biochemistry 43, 6535 (2004), Abstract
Kinetic and mechanistic studies on the hydrolysis of ubiquitin C-terminal 7-amido-4-methylcoumarin by deubiquitinating enzymes: L. C. Dang et al.; Biochemistry 37, 1868 (1998), Abstract

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Ubiquitin-AMC

BML-SE211

Product Details

Handling & Storage

Datasheet, Manuals, SDS & CofA

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