Highly sensitive, well-validated ELISA kit with results in <3h
- High sensitivity measurement, detecting as little as 10.54 pg/ml of TXB2
- High throughput format with results in < 3 hours for up to 37 samples in duplicate
- Widely cited in peer-reviewed publications in a diverse assortment of sample types including mucosa, tissue, serum, plasma, urine, and much more
- Reliable and reproducible results day-after-day and lot-after-lot
The TXB2 ELISA kit is a colorimetric competitive enzyme immunoassay kit with results in < 3 hours. Absorbance is read at 405 nm. Detect COX I inhibition by measuring the production of downstream TXB2 in a convenient 96 well plate based assay. This kit is highly cited in peer-reviewed publications in a diverse assortment of sample types including mucosa, tissue, serum, plasma, urine, and much more. The TXB2 ELISA kit provides easy-to-use stable liquid color-coded reagents that reduce errors.
Thromboxane A2 (TXA2) is involved in platelet aggregation, vasoconstriction and reproductive functions, but has a half life of only 37 seconds under physiological conditions. Thromboxane B2 (TXB2) is the stable product of the non-enzymatic hydration of TXA2, thus the production of TXA2 in vivo is typically monitored by measurement of TXB2 and its metabolite 2,3-dinor TXB2. 11-dehydro-TXB2 is a major metabolite of Thromboxane B2 (TXB2) found in urine and plasma produced by its dehydrogenation by the enzyme 11-OH-dehydrogenase.
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Product Details
Alternative Name |
Thromboxane B2 |
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Application |
Colorimetric detection, ELISA |
Application Notes |
For the quantitative determination of TXB2 in culture supernatants, plasma, serum, saliva, and urine from any species. Cited sample types include cell lysate, coronary effluent, liver perfusate, platelets, and whole blood. |
Assay Time |
<3 hours |
Compatibility |
This product is compatible with the Absorbance 96 Plate Reader. |
Contents |
GxR IgG Microtiter plate, Conjugate, Antibody, Assay buffer, Wash buffer concentrate, Standard, pNpp Substrate, Stop solution |
Sensitivity |
10.54 pg/ml (range 13.7 – 10,000 pg/ml) |
Species Reactivity |
Species independent |
Wavelength |
405 nm |
Handling & Storage
Long Term Storage |
+4°C |
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Shipping |
Blue Ice |
Regulatory Status |
RUO – Research Use Only |
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- An optimized herbal medicine containing Scutellaria baicalensis Georgi, Alisma orientale Juzepzuk, and Atractylodes japonica Koidzumi has potent antiplatelet and antithrombotic activities: Y.J. Kim, et al.; J. Tradit. Complement. Med. 13, 285 (2023), Abstract
- Antiplatelet Effect of Daphnetin Is Regulated by cPLA2-Mediated Thromboxane A2 Generation in Mice: P.K. Chaudhary, et al.; Int. J. Mol. Sci. 24, 5779 (2023), Abstract
- Soluble epoxide hydrolase maintains steady-state lipid turnover linked with autocrine signaling in peritoneal macrophages: Liu, F., Diao, X., et al.; iScience 26, 107465 (2023), Abstract
- Blocking of PI3-kinase beta protects against cerebral ischemia/reperfusion injury by reducing platelet activation and downstream microvascular thrombosis in rats: Q. Cheng, et al.; Sci. Rep. 13, 2030 (2023), Abstract
- Dietary sodium restriction prevents vascular endothelial growth factor inhibitor-induced hypertension: L. van Doorn, et al.; Br. J. Cancer 128, 354 (2023), Abstract
- Differential effects of cyclo-oxygenase 1 and 2 inhibition on angiogenesis inhibitor-induced hypertension and kidney damage: K.M. Mirabito Colafella, et al.; Clin. Sci. (Lond.) 136, 675 (2022), Abstract
- Aspirin Inhibits the Inflammatory Response of Protease-Activated Receptor 1 in Pregnancy Neutrophils: Implications for Treating Women with Preeclampsia: Walsh, S. W., Al Dulaimi, M., et al.; Int. J. Mol. Sci. 23, (2022), Abstract
- Sex-related differences in the response of anti-platelet drug therapies targeting purinergic signaling pathways in sepsis: Amoafo, E. B., Entsie, P., et al.; Front. Immunol. 13, 1015577 (2022), Abstract
- Infection of Galleria mellonella (lepidoptera) larvae with the entomopathogenic fungus Conidiobolus coronatus (entomophthorales) induces apoptosis of hemocytes and affects the concentration of eicosanoids in the hemolymph: A.K. Wronska, et al.; Front. Physiol. 12, 774086 (2022), Application(s): ELISA kit using hemolymph, Abstract — Full Text
- Chronic edible dosing of Δ9-tetrahydrocannabinol (THC) in nonhuman primates reduces systemic platelet activity and function: S.E. Reitsma, et al.; Am. J. Physiol. Cell Physiol. 322, 370 (2022), Abstract
- Pharmacokinetic and pharmacodynamic profiles of a novel phospholipid-aspirin complex liquid formulation and low dose enteric-coated aspirin: results from a prospective, randomized, crossover study: F. Franchi, et al.; J. Thromb. Thrombolysis 54, 373 (2022), Abstract
- Antioxidants and/or fish oil reduce intermittent hypoxia-induced inflammation in the neonatal rat terminal ileum: G. Mustafa, et al.; Prostaglandins Other Lipid Mediat. 155, 106565 (2021), Abstract
- Effect of the Renin-Angiotensin-Aldosterone System Reactivity on Endothelial Function and Modulative Role of Valsartan in Male Subjects with Essential Hypertension: Jasiczek, J., Trocha, M., et al.; J. Clin. Med. 10, (2021), Abstract
- Unexpected effects of long-term treatment with acetylsalicylic acid on late phase of pulmonary metastasis in murine model of orthotopic breast cancer: M. Smeda, et al.; PLoS One 15, e0230520 (2020), Abstract — Full Text
- Effects of intestinal flora on the pharmacokinetics and pharmacodynamics of aspirin in high-altitude hypoxia: Y. Sun, et al.; PLoS One 15, e0230197 (2020), Abstract — Full Text
- Redundant role of ASK1-mediated p38MAPK activation in human platelet function: K.M. Sledz, et al.; Cell. Signal. 68, 109528 (2020), Abstract
- The protein tyrosine phosphatase PTPN7 is a negative regulator of ERK activation and thromboxane generation in platelets: Inamdar, V. V., Reddy, H., et al.; J. Biol. Chem. 294, 12547 (2019), Abstract
- Critical roles for the phosphatidylinositide 3-kinase isoforms p110β and p110γ in thrombopoietin-mediated priming of platelet function: S.F. Moore, et al.; Sci. Rep. 9, 1468 (2019), Application(s): ELISA- Mouse Platelet Supernatant, Abstract — Full Text
- Endothelin-1-Induced Microvascular ROS and Contractility in Angiotensin-II-Infused Mice Depend on COX and TP Receptors: C.S. Wilcox, et al.; Antioxidants (Basel) 8, E193 (2019), Abstract — Full Text
- Molecular profiling and functional analysis of macrophage-derived tumor extracellular vesicles: C. Cianciaruso, et al.; Cell Rep. 27, 3062 (2019), Application(s): ELISA using culture supernatants, Abstract — Full Text
- Ligustrazine inhibits platelet activation via suppression of the Akt pathway: L. Li, et al.; Int. J. Mol. Med. 43, 575 (2019), Abstract
- Hyperglycemia enhances constriction of retinal venules via activation of the reverse-mode sodium-calcium exchanger: Y.L. Chen, et al.; Diabetes 68, 1624 (2019), Application(s): ELISA using pig vitreous humor, Abstract
- A comparative study on the effect of argan oil versus fish oil on risk factors for cardio-vascular disease in high-fat-fed rats: A. Haimeur, et al.; Nutrition 57, 32 (2019), Application(s): ELISA using rat platelets, Abstract
- Inhibitory Effects of an Orally Active Thromboxane A2 Receptor Antagonist, nstpbp5185, on Atherosclerosis in ApoE-Deficient Mice: S.W. Huang, et al.; Thromb. Haemost. 118, 401 (2018), Abstract
- NRF2 prevents hypertension, increased ADMA, microvascular oxidative stress, and dysfunction in mice with two weeks of ANG II infusion: C. Wang, et al.; Am. J. Physiol. Regul. Integr. Comp. Physiol. 314, R399 (2018), Application(s): ELISA of mouse urine, Abstract — Full Text
- Protective Effects of Total Flavones of Elaeagnus rhamnoides (L.) A. Nelson against Vascular Endothelial Injury in Blood Stasis Model Rats: Z. Wei, et al.; Evid. Based Complement. Alternat. Med. 2017, 8142562 (2017), Abstract — Full Text
- Effect of Regular Aerobic Activity in Young Healthy Athletes on Profile of Endothelial Function and Platelet Activity: K. Podgórska, et al.; BioMed Res. Int. 2017, 8715909 (2017), Application(s): Human plasma, Abstract — Full Text
- Dietary acrylamide exposure in male F344 rats: Dataset of systemic oxidative stress and inflammation markers: X. Jin, et al.; Data Brief 7, 460 (2016), Application(s): Measured TXB2 in serum samples, Abstract
- Moderate intake of docosahexaenoic acid raises plasma and platelet vitamin E levels in cystic fibrosis patients: E. Vericel, et al.; Prostaglandins Leukot. Essent. Fatty Acids 115, 41 (2016), Application(s): Basal formation of platelet thromboxane B2 and its urinary 11-dehydro metabolite, Abstract — Full Text
- Local intra-uterine Ang-(1-7) infusion attenuates PGE2 and 6-keto PGF1α in decidualized uterus of pseudopregnant rats: K.B. Brosnihan, et al.; Reprod. Biol. Endocrinol. 14, 68 (2016), Application(s): Assaying tissue extracts, Abstract — Full Text
- Water soluble tomato concentrate regulates platelet function via the mitogen-activated protein kinase pathway: D. Jeong, et al.; Korean J. Vet. Res. 56, 67 (2016), Application(s): Measurement of TXB2 production effect, Full Text
- The lack of aspirin resistance in patients with coronary artery disease: N. Homorodi, et al.; J. Transl. Med. 14, 74 (2016), Application(s): Detection of aspirin effect, Abstract — Full Text
- Cardiac volume overload and pulmonary hypertension in long-term follow-up of patients with a transjugular intrahepatic portosystemic shunt: A. Wannhoff, et al.; Aliment. Pharmacol. Ther. 43, 955 (2016), Application(s): Measured peripheral blood levels of thromboxane B2 (TXB2), Abstract — Full Text
- Nanoparticle biointerfacing by platelet membrane cloaking: C.J. Hu, et al.; Nature 526, 118 (2015), Application(s): Human platelet aggregation assay, Abstract
- Aspirin resistance in adult patients after Fontan surgery: L. Tomkiewicz-Pajak, et al.; Int. J. Cardiol. 181, 19 (2015), Application(s): Human Plasma, Abstract
- Moderate oral supplementation with docosahexaenoic acid improvesplatelet function and oxidative stress in type 2 diabetic patients: E. Véricel, et al.; Thromb. Haemost. 114, 289 (2015), Application(s): Human Urine, Abstract
- Endothelial dysfunction in young healthy men is associated with aspirin resistance: A. Doroszko, et al.; Vascul. Pharmacol. 67-69, 30 (2015), Application(s): ELISA using human blood, Abstract
- Phosphatidylinositol-3,4,5-trisphosphate stimulates Ca2+ elevation and Akt phosphorylation to constitute a major mechanism of thromboxane A2 formation in human platelets: N. Kassouf, et al.; Cell Signal. 27, 1488 (2015), Application(s): ELISA using human platelets, Abstract
- Pistacia chinensis Methanolic Extract Attenuated MAPK and Akt Phosphorylations in ADP Stimulated Rat Platelets In Vitro: J.Y. Park, et al.; Evid. Based Complement. Alternat. Med. 2012, 895729 (2012), Application(s): EIA using rat cell lysates, Abstract — Full Text
- Time-dependent changes in non-COX-1-dependent platelet function with daily aspirin therapy: D. Voora, et al.; J. Thromb. Thrombolysis 33, 246 (2012), Application(s): ELISA using human serum, Abstract — Full Text
- Delta-6-desaturase (FADS2) deficiency unveils the role of ω3-and ω6-polyunsaturated fatty acids: W. Stoffel, et al.; EMBO J. 27, 2281 (2008), Application(s): EIA using mouse culture supernatants, Abstract
- Discovery of ecopladib, an indole inhibitor of cytosolic phospholipase A2 alpha: K. Lee, et al.; J. Med. Chem. 50, 1380 (2007), Application(s): EIA using rat & human whole blood, Abstract
- Altered kidney graft high-energy phosphate metabolism in kidney-transplanted end-stage renal disease type 1 diabetic patients – A cross-sectional analysis of the effect of kidney alone and kidney-pancreas transplantation: P. Fiorina, et al.; Diabetes Care 30, 597 (2007), Application(s): EIA using human urine, Abstract
- Role of thromboxane A2 in early BDL-induced portal hypertension: Y. Yokoyama, et al.; Am. J. Physiol. Gastrointest. Liver Physiol. 284, G453 (2003), Application(s): EIA using liver perfusate, Abstract — Full Text
- Conjugated linoleic acid exhibits stimulatory and inhibitory effects on prostanoid production in human endothelial cells and platelets: A.P. Torres-Duarte & J.Y. Vanderhoek; Biochim. Biophys. Acta. 1640, 69 (2003), Application(s): EIA using platelets, Abstract
- The roles of alpha IIb beta 3-mediated outside-in signal transduction, thromboxane A2, and adenosine diphosphate in collagen-induced platelet aggregation: M.J. Cho, et al.; Blood 101, 2646 (2003), Application(s): EIA using mouse plasma, Abstract
- Role of the Src family kinase Lyn in TxA2 production, adenosine diphosphate secretion, Akt phosphorylation, and irreversible aggregation in platelets stimulated with gamma-thrombin: M.J. Cho, et al.; Blood 99, 2442 (2002), Application(s): EIA using mouse platelets, Abstract
- Prevention of influenza-induced lung injury in mice overexpressing extracellular superoxide dismutase: H.B. Suliman, et al.; Am. J. Physiol. Lung Cell Mol. Physiol. 280, L69 (2001), Application(s): EIA using cell lysate, Abstract — Full Text
- A high level of cyclooxygenase-2 inhibitor selectivity is associated with a reduced interference of platelet cyclooxygenase-1 inactivation by aspirin: M. Ouellet, et al.; PNAS 98, 14583 (2001), Application(s): EIA using human culture supernatants, Abstract
- Sevoflurane and Isoflurane Do Not Enhance the Pre- and Postischemic Eicosanoid Production in Guinea Pig Hearts: B. Heindl, et al.; Anesth. Analg. 90, 17 (2000), Application(s): EIA using guinea pig coronary effluent, Abstract
- Streptokinase-induced platelet activation involves antistreptokinase antibodies and cleavage of protease-activated receptor-1: J.P. McRedmond, et al.; Blood 95, 1301 (2000), Application(s): EIA using human plasma, Abstract
- A Sequence within the Cytoplasmic Tail of GpIIb Independently Activates Platelet Aggregation and Thromboxane Synthesis: G. Stephens, et al.; J. Biol. Chem. 273, 20317 (1998), Application(s): EIA using human cell lysates, Abstract
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