TUBE 2 (His6)

Based on protein domains known to possess an affinity for ubiquitin, Tandem Ubiquitin Binding Entities (TUBEs) have been developed for the isolation and identification of ubiquitinated proteins. TUBEs display up to a 1000-fold increase in affinity for poly-ubiquitin moieties over the single ubiquitin binding associated domain (UBA). In addition, TUBEs display a protective effect on polyubiquitinated proteins, allowing for detection at relatively low abundance. These properties effectively “capture” proteins in their polyubiquitylated state. The ubiquitylation state of certain cellular proteins can be directly related to various diseases including neurodegeneration, inflammation, and cancer. However, ubiquitylated proteins are intrinsically unstable, which precludes the characterization of the ubiquitinylation state in many cases. TUBEs will fill this void in ubiquitin research, allowing the identification of ubiquitin-modified proteins that cannot be detected with current technologies. TUBEs can be used to isolate, enrich, and identify ubiquitylated proteins from cells, tissues and organs. TUBE 2 is based on UBA1 from human RAD23A. The affinity of TUBE 2 for K63 linked tetra-ubiquitin and K48 tetra-ubiquitin linked chains is equivalent.