TUBE 1 (His6)

Based on protein domains known to possess an affinity for ubiquitin, Tandem Ubiquitin Binding Entities (TUBEs) have been developed for the isolation and identification of ubiquitinated proteins. TUBEs display up to a 1000-fold increase in affinity for poly-ubiquitin moieties over the single ubiquitin binding associated domain (UBA). In addition, TUBEs display a protective effect on polyubiquitinated proteins, allowing for detection at relatively low abundance. These properties effectively “capture” proteins in their polyubiquitinated state. The ubiquitination state of certain cellular proteins can be directly related to various diseases including neurodegeneration, inflammation and cancer. However, ubiquitinated proteins are intrinsically unstable, which precludes the characterization of the ubiquitinylation state in many cases. TUBEs will fill this void in ubiquitin research, allowing the identification of ubiquitin-modified proteins that cannot be detected with current technologies. TUBEs can be used to isolate, enrich, and identify ubiquitylated proteins from cells, tissues, and organs. TUBE 1 is based on UBAs from the protein ubiquilin.