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TRIF polyclonal antibody Western blot
Poly(I:C) activates the TLR3/TRIF signaling.(A) HLECs were stimulated with Poly(I:C) at 0.1, 1 and 10 μg/mL for 3, 6, 12 and 24 h. Whole cell lysates were analyzed for TLR3, TRIF, phosphorylated-IRF3, total IRF3, IκBα, phosphorylated-NF-κB, NF-κB, RIG-I, MDA5, and β-tubulin (as a loading control) by Western blot. Arrowheads indicate TLR3 (116 kDa, lower band) and TRIF (76 kDa, top band). #, indicates a nonspecific band. Immunoblots are representative of three separate experiments that all showed similar results. (B) Poly(I:C) induces nuclear translocation of NF-κB. HLEC were stimulated with medium (NT) or 5 μg/mL Poly(I:C) for 24 h. Immunofluorescence staining was performed using antibodies against NF-κB (red) and nuclei were counterstained with Hoechst 33342 (blue). Images are representative of three separate experiments (400x total magnification).
Image collected and cropped by CiteAb under a CC0-1.0 license from the following publication: Poly(I:C) Induces Human Lung Endothelial Barrier Dysfunction by Disrupting Tight Junction Expression of Claudin-5. PLoS One (2016)
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RUO – Research Use Only |
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