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Transglutaminase Colorimetric Microassay Kit

CVL-OPR0001

Axxora Marketplace
  • CVL-OPR0001   —   3 plates
    $845.00

Principle of the assay:

The TG-Covtest uses Z-Gln-Gly as the first substrate (amine acceptor) and biotin cadaverine as second (amine donor). In the 1st step, samples suspected of containing TGase are incubated with calcium, dithiothreitol (DTT) and biotinylated cadaverine in the wells of microtiter plates to which Z-Gln-Gly had been covalently coupled previously. In the presence of TGase biotinylated cadaverine is incorporated onto the γ-carboxamide of the glutaminyl residue of the dipeptide to form γ-glutamyl cadaverine biotin. In the second step streptavidin-labelled peroxidase is added to the wells. In the third step, peroxidase activity is revealed using H2O2 as HRP substrate and tetramethyl benzidine as electron acceptor (chromogen).

The TG-Covtest has two original features:
– The use of covalently coupled Z-Gln-Gly solves the problem of the absorbed substrate leaching out.
– The second feature is that 96 samples can be simultaneously screened in 30 minutes.

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