Transglutaminase 2 (rat) (recombinant) (His)

Add 10mM Ca2+ to activate rat TG2.>750 U/mg [Activity is determined by measuring the rate of fluorescence enhancement after His6-rrTG2-catalyzed monodansylcadaverine-incorporation into N,N-dimethylated casein according to Lorand et al., Anal. Biochem. 44 (221-231). 1 U is defined as the increase in fluorescence intensity of 1 a.u./min (measured on a Cary eclipse fluorescence spectrophotometer, Varian; λex=332 nm, λem=500 nm; band filter =5 nm; detector strength =600 V; temperature =37°C, assay volume =1 ml)].

Tissue-type Transglutaminase, TG2, TGase 2, Protein-glutamine-γ-glutamyltransferase

White solid.

Lyophilized from 50mM NaH₂PO₄, pH 8.0, containing 150mM sodium chloride. Sample contains maltodextrin.

~77kDa

≥95% (SDS-PAGE)

Reconstitute with water. Rotate vial gently until solid dissolves.

Based on the clone IRBPp993H102D. It is N-terminally fused to a His₆-tag.

Produced in E.coli.

For the Original Manufacturer’s data sheet please click here.