Transglutaminase 2 (human) (recombinant) (His)

Tissue-type Transglutaminase, TG2, TGase 2, Protein-glutamine-γ-glutamyltransferase

White solid.

Lyophilized from 50mM NaH₂PO₄, pH 8, containing 150mM NaCl, maltodextrin and <0.1mM imidazole.

~78kDa.

≥90% (SDS-PAGE).

Reconstitute with 160µl water. For activation add 10mM Ca2+.

Produced in E. coli.

<750U/mg protein. One unit is defined as the increase of fluorescence intensify of 1 a.u./min. Activity is determined by measuring the rate of fluorescence enhancement after TG2-catalyzed monodansylcadaverine-incorporation into N, N-dimethylated casein (L. Lorand, et al.; Anal. Biochem. 44, 221 (1971)).

For the Original Manufacturer’s data sheet please click here.