AMPIVIEW® RNA probes
Enabling Your Projects 
GMP Services
Bulk Solutions
Research Travel Grant
Have You Published Using an Enzo Product?
Shipping: Available products typically ship within 24/48h, via priority shipping.
Do you need support? Contact Customer Service or Technical Support.
Online Account
Access or Create Your Account
SDS PAGE Analysis: Lane 1: MW Marker, Lane 2: 1μg TRAIL.
Product Details
| Alternative Name |
Apo-2L, TNFSF 10, CD253 |
|---|---|
| Application Notes |
ELISA: binds to human TRAIL receptors and human osteoprotegerin (OPG) at 10-100 ng/ml. |
| Biological Activity |
Induces apoptosis if applied with the cross-linking enhancer (Prod. No. ALX-804-034); see also TRAIL, Soluble (human) (recombinant) Set (Prod. No. ALX-850-018). Note: rhsTRAIL does not significantly induce apoptosis in the absence of an enhancer. |
| Concentration |
0.1mg/ml after reconstitution. |
| Endotoxin Content |
<0.1EU/µg purified protein (LAL test; Associates of Cape Cod). |
| Formulation |
Lyophilized. Contains PBS. |
| MW |
~28kDa |
| Purity |
≥90% (SDS-PAGE) |
| Reconstitution |
Reconstitute with 100µl sterile water. Further dilutions should be made with medium containing 5% fetal calf serum. |
| Source |
Produced in E. coli. The extracellular domain of human TRAIL (aa 95-281) is fused at the N-terminus to a linker peptide (8 aa) and a FLAG®-tag. |
| Specificity |
Binds to human and mouse TRAIL receptors and human osteoprotegerin (OPG). |
| Technical Info / Product Notes |
Historical data has shown that Fas induces apoptosis in a concentration range of 1-100ng/ml if applied with the cross-linking enhancer (Prod. No. ALX-804-034). |
| UniProt ID |
P50591 |
Handling & Storage
| Use/Stability |
Stable for at least 6 months after receipt when stored at -20°C. |
|---|---|
| Handling |
Avoid freeze/thaw cycles. After reconstitution, prepare aliquots and store at -20°C. |
| Long Term Storage |
-20°C |
| Shipping |
Blue Ice |
| Regulatory Status |
RUO – Research Use Only |
|---|
- XIAP promotes melanoma growth by inducing tumour neutrophil infiltration: M. Daoud, et al.; EMBO Rep. 23, e53608 (2022), Abstract
- The non-apoptotic function of Caspase-8 in negatively regulating the CDK9-mediated Ser2 phosphorylation of RNA polymerase II in cervical cancer: R. Mandal, et al.; Cell. Mol. Life Sci. 79, 597 (2022), Abstract
- Anti-PD-1 antibody increases NK cell cytotoxicity towards nasopharyngeal carcinoma cells in the context of chemotherapy-induced upregulation of PD-1 and PD-L1: A. Makowska, et al.; Cancer Immunol. Immunother. 70, 323 (2021), Abstract — Full Text
- BH3-only protein expression determines hepatocellular carcinoma response to sorafenib-based treatment: S. Busche, et al.; Cell Death Dis. 12, 736 (2021), Abstract
- Caspase-9 acts as a regulator of necroptotic cell death: Molnár, T., Pallagi, P., et al.; FEBS J. 288, 6476 (2021), Abstract
- The Mitochondrial Ca2+ Overload via Voltage-Gated Ca2+ Entry Contributes to an Anti-Melanoma Effect of Diallyl Trisulfide: C. Nakagawa, et al.; Int. J. Mol. Sci. 21, 491 (2020), Abstract — Full Text
- Regulation of the unfolded protein response through ATF4 and FAM129A in prostate cancer: Pällmann, N., Livgård, M., et al.; Oncogene 38, 6301 (2019), Abstract
- Higher-Order Clustering of the Transmembrane Anchor of DR5 Drives Signaling: Pan, L., Fu, T. M., et al.; Cell 176, 1477 (2019), Abstract
- Redundant and receptor-specific activities of TRADD, RIPK1 and FADD in death receptor signaling: S. Fullsack, et al.; Cell Death Dis. 10, 122 (2019), Application(s): TRAIL treatment to stimulate TRAIL-related apoptotic gene expression, Abstract — Full Text
- Inhibition of Notch1 signaling overcomes resistance to the death ligand Trail by specificity protein 1-dependent upregulation of death receptor 5: A. Fassl, et al.; Cell Death Dis. 6, e1921 (2015), Abstract — Full Text
- Death receptor 5 signaling promotes hepatocyte lipoapoptosis: S.C. Cazanave, et al.; J. Biol. Chem. 286, 39336 (2011), Abstract
- A role for cFLIP in B cell proliferation and stress MAPK regulation: H. Zhang, et al.; J. Immunol. 182, 207 (2009), Abstract — Full Text
- Silencing of prion protein sensitizes breast adriamycin-resistant carcinoma cells to TRAIL-mediated cell death: F. Meslin, et al.; Cancer Res. 67, 10910 (2007), Abstract
- Cytoskeleton-mediated death receptor and ligand concentration in lipid rafts forms apoptosis-promoting clusters in cancer chemotherapy: C. Gajate & F. Mollinedo; J. Biol. Chem. 280, 11641 (2005), Abstract — Full Text
- NF-κB signals induce the expression of c-FLIP: O. Micheau, et al.; Mol. Cell. Biol. 21, 5299 (2001), Abstract — Full Text
- The cytokines tumor necrosis factor α (TNF-α) and TNF-related apoptosis-inducing ligand differentially modulate proliferation and apoptotic pathways in human keratinocytes expressing the h: J.R. Basile, et al.; J. Biol. Chem. 276, 22522 (2001), Abstract — Full Text
- Tumor necrosis factor (TNF) and phorbol ester induce TNF-related apoptosis-inducing ligand (TRAIL) under critical involvement of NF-kappa B essential modulator (NEMO)/IKKgamma: D. Siegmund, et al.; J. Biol. Chem. 276, 43708 (2001), Abstract — Full Text
- Combined effect of tumor necrosis factor-related apoptosis-inducing ligand and ionizing radiation in breast cancer therapy: A.M. Chinnaiyan, et al.; PNAS 97, 1754 (2000), Abstract
- FADD/MORT1 and caspase-8 are recruited to TRAIL receptors 1 and 2 and are essential for apoptosis mediated by TRAIL receptor 2: M.R. Sprick, et al.; Immunity 12, 599 (2000), Abstract
- Fas triggers an alternative, caspase-8-independent cell death pathway using the kinase RIP as effector molecule: N. Holler, et al.; Nat. Immunol. 1, 489 (2000), Abstract
- Conversion of membrane-bound Fas(CD95) ligand to its soluble form is associated with downregulation of its proapoptotic activity and loss of liver toxicity: P. Schneider, et al.; J. Exp. Med. 187, 1205 (1998), Abstract
- Characterization of two receptors for TRAIL: P. Schneider, et al.; FEBS Lett. 416, 329 (1997), Abstract
- Inhibition of death receptor signals by cellular FLIP: M. Irmler, et al.; Nature 388, 190 (1997), Abstract
- Viral FLICE-inhibitory proteins (FLIPs) prevent apoptosis induced by death receptors: M. Thome, et al.; Nature 386, 517 (1997), Abstract
Related Products
Enhancer for Ligands
ALX-804-034
Enhances biological activity of TRAIL, soluble (human) (recombinant) and FasL, soluble (human) (recombinant).
Datasheet, Manuals, SDS & CofA
Manuals And Inserts
Certificate of Analysis
Please enter the lot number as featured on the product label
SDS
Enzo Life Science provides GHS Compliant SDS
If your language is not available please fill out the SDS request form