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This antibody is covered by our Worry-Free Guarantee.

Figure 3: Immunostaining of HEK 293T cells transfected with a expression plasmid coding for human TRAIL (left panels), or mock transfected (right panel).Method: 3 days after transfection of cells as indicated, cells were fixed with 4% formaldehyde or acetone for 5 min. at RT. Slides were blocked with IgG, and incubated for 1 hour with PAb to TRAIL (Prod. No. ALX-210-732) at a dilution of 1:100 or control rabbit IgG in PBS containing 1% BSA. After washes with PBS, samples were incubated with a biotinylated secondary antibody to rabbit IgG for 1 hour, washed in PBS and revealed with StreptABComplex/HRP (Vector) and AEC.”””,TRAIL (human) polyclonal antibody Immunocytochemistry,TRAIL (human) polyclonal antibody Immunocytochemistry,yes
ALX-210-732,210-732_immunogen.gif,http://static.enzolifesciences.com/fileadmin/files/image/210-732_immunogen.gif,Figure 1: Schematic structure of the immunogen.,TRAIL (human) polyclonal antibody Schematic structure of immunogen,TRAIL (human) polyclonal antibody Schematic structure of immunogen,yes
ALX-210-732,210-732.gif,http://static.enzolifesciences.com/fileadmin/files/image/210-732.gif,Figure 2: Detection of recombinant human TRAIL (Prod. No. ALX-522-003) but not of recombinant human FasL (Prod. No. ALX-522-001). Method: Recombinant soluble TRAIL

Figure 2: Detection of recombinant human TRAIL (Prod. No. ALX-522-003) but not of recombinant human FasL (Prod. No. ALX-522-001). Method: Recombinant soluble TRAIL, and recombinant soluble FasL as negative control, were resolved by SDS-PAGE under reducing conditions, transferred to nitrocellulose and detected with PAb to TRAIL (human) (Prod. No. ALX-210-732) at a dilution of 1:500. Proteins were visualized using a peroxidase-conjugated antibody to rabbit IgG and a chemiluminescence detection system.



Product Details
Alternative Name |
Apo-2L, TNFSF 10, CD253 |
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Application |
Flow Cytometry, ICC, IHC (FS), WB |
Crossreactivity |
Does not cross-react with mouse TRAIL. |
Formulation |
Liquid. In PBS containing 0.02% sodium azide. |
GenBank ID |
U57059 |
Gene/Protein Identifier |
8743 (Entrez GeneID) |
Host |
Rabbit |
Immunogen |
Recombinant human soluble TRAIL (aa 95-281). |
Purity Detail |
Protein A-affinity purified. |
Source |
Purified from serum. |
Species Reactivity |
Human |
Specificity |
Recognizes soluble TRAIL. |
UniProt ID |
P50591 |
Worry-free Guarantee |
This antibody is covered by our Worry-Free Guarantee. |
Handling & Storage
Use/Stability |
Stable for at least 1 year after receipt when stored as recommended. |
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Handling |
Avoid freeze/thaw cycles. |
Long Term Storage |
+4°C |
Shipping |
Blue Ice |
Regulatory Status |
RUO – Research Use Only |
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- Inhibition of ATM kinase upregulates levels of cell death induced by cannabidiol and γ-irradiation in human glioblastoma cells.: Ivanov, V. N., Wu, J., et al.; Oncotarget 10, 825 (2019), Application(s): FC/FACS / Reactant(s): Human, Abstract
- Regulation of human glioblastoma cell death by combined treatment of cannabidiol, γ-radiation and small molecule inhibitors of cell signaling pathways.: Hei, T. K., Wu, J., et al.; Oncotarget 8, 74068 (2017), Application(s): FC/FACS / Reactant(s): Human, Abstract
- Methanolic extract of white asparagus shoots activates TRAIL apoptotic death pathway in human cancer cells and inhibits colon carcinogenesis in a preclinical model: Bousserouel, S., Le Grandois, J., et al.; Int. J. Oncol. 43, 394 (2013), Abstract
- Tumor-associated B7-H1 promotes T-cell apoptosis: A potential mechanism of immune evasion: H. Dong, et al.; Nat. Med. 8, 793 (2002), Abstract
- Hypericin photo-induced apoptosis involves the tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) and activation of caspase-8: C.M. Schempp, et al.; FEBS Lett. 493, 26 (2001), Abstract
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Datasheet, Manuals, SDS & CofA
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SDS
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