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TRAIL (human) monoclonal antibody (2E5)

ALX-804-296

11 citations

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This antibody is covered by our Worry-Free Guarantee.

TRAIL (human) monoclonal antibody (2E5) Flow Cytometry — **Figure 1:** Apoptosis induced in Jurkat T leukemia cells by *Killer*TRAIL^TM (Prod. No. ALX-201-073) is completely blocked by mouse MAb to TRAIL (human) (2E5) (Prod. No. ALX-804-296) in a concentration range of 0.5-1.5µg/ml. Concentration of MAb 2E5 required for inhibition may vary depending of the cell type studied and the concentration of soluble recombinant human TRAIL used. The neutralizing activity of MAb 2E5 has been confirmed with various sources of soluble recombinant human TRAIL including *Killer*TRAIL^TM.Method: Jurkat cells (5×10⁴) were treated with *Killer*TRAIL^TM (300ng/ml) in the presence or absence of the MAb to TRAIL (human) (2E5) or a control IgG1 mouse monoclonal antibody. After 16 hours apoptosis was evaluated by forward/sideward scatter (FSC/SSC) combined with propidium iodide (PI) staining analyzed by flow cytometry.”””””

TRAIL-R1 (human) monoclonal antibody (HS101) (FITC conjugate) Flow Cytometry

TRAIL (human) monoclonal antibody (2E5) Functional Application Neutralization — **Figure:** Neutralizing effect of MAb to TRAIL (human) (2E5) on TRAIL-induced apoptosis (TRAIL, Soluble (human) (recombinant) Set (Prod. No. ALX-850-018); containing Enhancer for Ligands).

Product Details

Alternative Name	Apo-2L, TNFSF 10, CD253
Application	Flow Cytometry, FUNC
Application Notes	Functional Application (excellent): neutralizes human TRAIL mediated killing in a concentration range of 0.5-1.5µg/ml. Only detects the native protein. Does not work in WB, ICC, IHC or other applications where fixation is necessary.
Clone	2E5
Crossreactivity	Does not cross-react with mouse TRAIL.
Formulation	Liquid. In PBS, pH 7.4. Contains no preservatives.
GenBank ID	U57059
Gene/Protein Identifier	8743 (Entrez GeneID)
Host	Mouse
Immunogen	Recombinant human soluble TRAIL (aa 95-281).
Isotype	IgG1
Purity Detail	Protein A-affinity purified. Sterile filtered.
Species Reactivity	Human
UniProt ID	P50591
Worry-free Guarantee	This antibody is covered by our Worry-Free Guarantee.

Handling & Storage

Short Term Storage	+4°C
Long Term Storage	+4°C
Shipping	Blue Ice

Regulatory Status	RUO – Research Use Only

Characterization of BV6-Induced Sensitization to the NK Cell Killing of Pediatric Rhabdomyosarcoma Spheroids: V. Särchen, et al.; Cells 12, 906 (2023), Abstract
Pediatric multicellular tumor spheroid models illustrate a therapeutic potential by combining BH3 mimetics with Natural Killer (NK) cell-based immunotherapy: V. Särchen, et al.; Cell Death Discov. 8, 11 (2022), Abstract
Interferon β and Anti-PD-1/PD-L1 Checkpoint Blockade Cooperate in NK Cell-Mediated Killing of Nasopharyngeal Carcinoma Cells: Makowska, A., Braunschweig, T., et al.; Transl. Oncol. 12, 1237 (2019), Abstract
A Bak-dependent mitochondrial amplification step contributes to Smac mimetic/glucocorticoid-induced necroptosis: K. Rohde, et al.; Cell Death Differ. 24, 83 (2017), Abstract
Inhibition of B-Raf/MEK/ERK signaling suppresses DR5 expression and impairs response of cancer cells to DR5-mediated apoptosis and T cell-induced killing: Y.T. Oh, et al.; Oncogene 35, 459 (2016), Application(s): Functional Studies, Abstract
NF-κB targeting by way of IKK inhibition sensitizes lung cancer cells to adenovirus delivery of TRAIL.: Griffith, T. S., Sanlioglu, S., et al.; BMC Cancer 10, 584 (2010), Application(s): FC/FACS / Reactant(s): Human, Abstract
Thalidomide induces limb anomalies by PTEN stabilization, Akt suppression, and stimulation of caspase-dependent cell death.: Schulze-Osthoff, K., Knobloch, J., et al.; Mol. Cell. Biol. 28, 529 (2008), Reactant(s): Chicken, Abstract
DR5 receptor mediates anoikis in human colorectal carcinoma cell lines: Laguinge, L. M., Samara, R. N., et al.; Cancer Res. 68, 909 (2008), Abstract
DcR2 (TRAIL-R4) siRNA and adenovirus delivery of TRAIL (Ad5hTRAIL) break down in vitro tumorigenic potential of prostate carcinoma cells: Sanlioglu, A. D., Karacay, B., et al.; Cancer Gene Ther. 14, 976 (2007), Abstract
IFN{alpha}-stimulated neutrophils and monocytes release a soluble form of TNF-related apoptosis-inducing ligand (TRAIL/Apo-2 ligand) displaying apoptotic activity on leukemic cells: C. Tecchio, et al.; Blood 103, 3837 (2004), Abstract

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TRAIL (human) monoclonal antibody (2E5)

ALX-804-296

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