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Total Phosphatidic Acid Assay Kit (Fluorometric)

CBI-MET-5019

Axxora Marketplace
  • CBI-MET-5019   —   100 assays
    $712.00
  • Detects total phosphatidic acid content (PA and LPA) in cell lysate samples
  • Detection sensitivity of approximately 5 uM phosphatidic acid
  • Phosphatidic Acid standard curve included

Our Total Phosphatidic Acid Assay Kit measures total phosphatidic acid content (PA and LPA) in cell lysate samples by a coupled enzymatic reaction system. First, lipase is used to hydrolyze phosphatidic acid to glycerol-3-phosphate. Next, the glycerol-3-phosphate product is oxidized by glycerol-3-phosphate oxidase (GPO), producing hydrogen peroxide. The hydrogen peroxide released from this reaction reacts specifically with the kit’s Fluorometric Probe and is detected at ex. 530-560 nm/em. 585-595 nm. Phosphatidic Acid levels in unknown sampled are determined based on the provided phosphatidic acid standard curve.

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Regulatory Status

RUO – Research Use Only