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Toll-like receptor 9 monoclonal antibody (26C593.2)

ALX-804-364

13 citations

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Toll-like receptor 9 monoclonal antibody (26C593.2) Western blot

Toll-like receptor 9 monoclonal antibody (26C593.2) Immunohistochemistry — Immunohistochemistry analysis: Formalin-fixed, paraffin-embedded human spleen probed with Toll-like receptor 9, mAb (26C593.2) at 5µg/ml.

Product Details

Alternative Name	TLR9, CD289
Application	Flow Cytometry, IHC (FS), IHC (PS), WB
Application Notes	Flow Cytometry: Suitable for cell surface and intracellular FC. Detects a band of ~120kDa by Western blot, may also detect a smaller band of TLR9 isoform B.
Clone	26C593.2
Formulation	Liquid. In PBS containing 0.05% BSA and 0.05% sodium azide.
Host	Mouse
Immunogen	Synthetic peptide corresponding to aa 268-284 in the cytoplasmic portion of human TLR9 (Toll-like Receptor 9).
Isotype	IgG1
Positive Control	Whole cell lysate from human peripheral blood monocyte cells (PBMC) or Daudi can be used as positive control.
Purity Detail	Protein G-affinity purified.
Recommendation Dilutions/Conditions	Flow Cytometry (intracellular, 0.1-2µg/106 cells)Immunohistochemistry (frozen sections, 10-20µg/ml)Western Blot (1-5µg/ml)Suggested dilutions/conditions may not be available for all applications.Optimal conditions must be determined individually for each application.
Species Reactivity	Dog, Horse, Human, Monkey, Mouse, Rat
UniProt ID	Q9NR96
Worry-free Guarantee	This antibody is covered by our Worry-Free Guarantee.

Handling & Storage

Handling	Avoid freeze/thaw cycles.
Short Term Storage	+4°C
Long Term Storage	-20°C
Shipping	Blue Ice

Regulatory Status	RUO – Research Use Only

Surface expression of Toll-like receptor 9 is upregulated on intestinal epithelial cells in response to pathogenic bacterial DNA: J.B. Ewaschuk, et al.; Infect. Immun. 75, 2572 (2007), Abstract
Intracellular signaling mechanisms regulating toll-like receptor-mediated activation of eosinophils: C.K. Wong, et al.; Am. J. Respir. Cell Mol. Biol. 37, 85 (2007), Abstract
Toll-like receptors in cellular subsets of human tonsil T cells: altered expression during recurrent tonsillitis: A. Mansson, et al.; Respir Res. 7, 36 (2006), Abstract — Full Text
Activation of anti-hepatitis C virus responses via Toll-like receptor 7: J. Lee, et al.; PNAS 103, 1828 (2006), Abstract — Full Text
Toll-like receptor 9 (TLR9) is present in murine liver sinusoidal endothelial cells (LSECs) and mediates the effect of CpG-oligonucleotides: M. Martin-Armas, et al.; J. Hepatol. 44, 939 (2006), Abstract
The Unc93b1 mutation 3d disrupts exogenous antigen presentation and signaling via Toll-like receptors 3, 7 and 9: K. Tabeta, et al.; Nat. Immunol. 7, 156 (2006), Abstract — Full Text
Expression of mRNA and proteins for toll-like receptors, associated molecules, defensins and LL-37 by SRIK-NKL, a CD8+ NK/T cell line: M.D. Srivastava and B.I. Srivastava; Leuk. Res. 29, 813 (2005), Abstract
TLR-induced inflammation in cystic fibrosis and non-cystic fibrosis airway epithelial cells: C.M. Greene, et al.; J. Immunol. 174, 1638 (2005), Abstract — Full Text
Human lung cancer cells express functionally active Toll-like receptor 9: D. Droemann, et al.; Respir. Res. 6, 1 (2005), Abstract — Full Text
TGF-alpha regulates TLR expression and function on epidermal keratinocytes: L.S. Miller, et al.; J. Immunol. 174, 6137 (2005), Abstract

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Toll-like receptor 9 monoclonal antibody (26C593.2)

ALX-804-364

Product Details

Handling & Storage

Datasheet, Manuals, SDS & CofA

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