TNF-α (human), (recombinant) (cell culture grade)

Tumor necrosis factor-α, TNFSF 2

Induces cytolysis of mouse L929 cells in the presence of 1µg/ml Actinomycin D (Prod. No. BML-GR300) (ED₅₀≤0.05ng/ml).

94948-59-1

<1EU/µg protein

Lyophilized from 0.2µm-filtered solution containing mannitol and trehalose.

14: 9812

~17kDa.

≥97% (SDS-PAGE)

A quick spin is recommended followed by reconstitution in distilled, sterile water to a concentration of 0.1-1mg/ml. For further dilution, add a carrier protein to avoid loss of biological activity.

Produced in yeast.

≥2×10⁷U/mg protein. Determined by standard cell cytotoxicity assay (N. Matthes & M.L. Neale; Lymphokines and Interferons, A Practical Approach; M.J. Clemens et al., eds.; IRL Press, p. 221).

P01375