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SDS-PAGE Analysis of TNF-α (soluble) (mouse), (recombinant). Lane 1: MW Marker, Lane 2: 1 µg TNF-α
Product Details
| Alternative Name |
Tumor necrosis factor-α, TNFSF 2 |
|---|---|
| Application Notes |
ELISA: binds to human TNF-R1 receptor. |
| Concentration |
1mg/ml after reconstitution. |
| Endotoxin Content |
<0.1EU/µg purified protein (LAL test; Associates of Cape Cod). |
| Formulation |
Lyophilized. Contains PBS. |
| Gene/Protein Identifier |
A002291 (UCSD Signaling Gateway ID) |
| MW |
~20kDa (SDS-PAGE). |
| Purity |
≥90% (SDS-PAGE) |
| Reconstitution |
Reconstitute with 50µl sterile water. Further dilutions should be made with medium containing 5% fetal calf serum. |
| Source |
Produced in E. coli. The extracellular domain of mouse TNF-α (tumor necrosis factor-α) (aa 77-235) is fused at the N-terminus to a linker peptide (8 aa) and a FLAG®-tag. |
| Specificity |
Binds to human, mouse and rat TNF-R1 and less efficiently to TNF-R2. In the presence of cross-linking enhancer (see Set Prod. No. ALX-850-061), TNF-α shows a significantly higher affinity for TNF-R2 than for TNF-R1, mimicking the characteristics of membrane-bound TNF-α. |
| Technical Info / Product Notes |
Historical lots have shown that it exerts its biological activity in a concentration range of 0.5-1ng/ml (WEHI 164 cells). FLAG is a registered trademark of Sigma-Aldrich Co. |
| UniProt ID |
P06804 |
Handling & Storage
| Use/Stability |
Stable for at least 6 months after receipt when stored at -20°C. |
|---|---|
| Handling |
Avoid freeze/thaw cycles. After reconstitution, prepare aliquots and store at -20°C. |
| Long Term Storage |
-20°C |
| Shipping |
Blue Ice |
| Regulatory Status |
RUO – Research Use Only |
|---|
- A RIPK1-specific PROTAC degrader achieves potent antitumor activity by enhancing immunogenic cell death: Mannion, J., Gifford, V., et al.; Immunity 57, 1514 (2024), Abstract
- ABIN1 (Q478) is Required to Prevent Hematopoietic Deficiencies through Regulating Type I IFNs Expression: Wu, X., Wang, Y., et al.; Adv. Sci. (Weinh.) 11, e2303555 (2024), Abstract
- Polarity protein AF6 functions as a modulator of necroptosis by regulating ubiquitination of RIPK1 in liver diseases: Xinyu, W., Qian, W., et al.; Cell Death Dis. 14, 673 (2023), Abstract
- Cleavage of cFLIP restrains cell death during viral infection and tissue injury and favors tissue repair: Martinez Lagunas, K., Savcigil, D. P., et al.; Sci. Adv. 9, eadg2829 (2023), Abstract
- Ubiquitin-binding domain in ABIN1 is critical for regulating cell death and inflammation during development: Li, M., Liu, Y., et al.; Cell Death Differ. 29, 2034 (2022), Abstract
- Synthesis of bioactive (1→6)-β-glucose branched poly-amido-saccharides that stimulate and induce M1 polarization in macrophages: Xiao, R., Zeng, J., et al.; Nat. Commun. 13, 4661 (2022), Abstract
- Bcl-3 promotes TNF-induced hepatocyte apoptosis by regulating the deubiquitination of RIP1: Hu, Y., Zhang, H., et al.; Cell Death Differ. 29, 1176 (2022), Abstract
- Tyrosine phosphorylation regulates RIPK1 activity to limit cell death and inflammation: Tu, H., Xiong, W., et al.; Nat. Commun. 13, 6603 (2022), Abstract
- RNPS1 inhibits excessive tumor necrosis factor/tumor necrosis factor receptor signaling to support hematopoiesis in mice: X. Zhong, et al.; PNAS 119, e2200128119 (2022), Abstract
- SMYD2 targets RIPK1 and restricts TNF-induced apoptosis and necroptosis to support colon tumor growth: Y.Q. Yu, et al.; Cell Death Dis. 13, 52 (2022), Abstract
- Simultaneous Inhibition of Three Major Cytokines and Its Therapeutic Effects: A Peptide-Based Novel Therapy against Endotoxemia in Mice: H.J. Shih, et al.; J. Pers. Med. 11, 436 (2021), Abstract
- Impaired RIPK1 ubiquitination sensitizes mice to TNF toxicity and inflammatory cell death: Kist, M., Komuves, L. G., et al.; Cell Death Differ. 28, 985 (2021), Abstract
- TAM Kinases Promote Necroptosis by Regulating Oligomerization of MLKL: Najafov, A., Mookhtiar, A. K., et al.; Mol. Cell 75, 457 (2019), Abstract
- Ubiquitination of RIPK1 suppresses programmed cell death by regulating RIPK1 kinase activation during embryogenesis: X. Zhang, et al.; Nat. Commun. 10, 4158 (2019), Abstract — Full Text
- K63-linked ubiquitination regulates RIPK1 kinase activity to prevent cell death during embryogenesis and inflammation: Y. Tang, et al.; Nat. Commun. 10, 4157 (2019), Abstract
- RIPK1 and Caspase-8 Ensure Chromosome Stability Independently of Their Role in Cell Death and Inflammation: G. Liccardi, et al.; Mol. Cell 73, 413 (2019), Abstract — Full Text
- Ubiquitin-Mediated Regulation of RIPK1 Kinase Activity Independent of IKK and MK2: A. Annibaldi, et al.; Mol. Cell 69, 566 (2018), Abstract — Full Text
- MK2 Phosphorylates RIPK1 to Prevent TNF-Induced Cell Death: I. Jaco, et al.; Mol. Cell 66, 698 (2017), Abstract — Full Text
- A novel TNFR1-triggered apoptosis pathway mediated by class IA PI3Ks in neutrophils: B. Geering, et al.; Blood 117, 5953 (2011), Application(s): Death assay on mouse neutrophils, Abstract — Full Text
- RasGAP-derived fragment N increases the resistance of beta cells towards apoptosis in NOD mice and delays the progression from mild to overt diabetes: N. Bulat, et al.; PLoS One 6, e22609 (2011), Abstract — Full Text
- Inhibition of interleukin 1 receptor/Toll-like receptor signaling through the alternatively spliced, short form of MyD88 is due to its failure to recruit IRAK-4: K. Burns, et al.; J. Exp. Med. 197, 263 (2003), Abstract — Full Text
- Conversion of membrane-bound Fas(CD95) ligand to its soluble form is associated with downregulation of its proapoptotic activity and loss of liver toxicity: P. Schneider, et al.; J. Exp. Med. 187, 1205 (1998), Abstract — Full Text
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