TNF-α (human), ELISA kit

• Highly sensitive ELISA, measuring as little as 8.4 pg/ml
• High throughput format with results for up to 39 samples in duplicate in just 4 hours
• Stable liquid color coded reagents and pre-coated plates reduce errors
• Fully quantitative results that surpass semi-quantitative Western blot analysis

The TNF-α (human), EIA kit is a colorimetric immunometric enzyme immunoassay kit with results in 4 hours. Absorbance is read at 450 nm. Assay up to 39 samples in duplicate per 96-well plate. The broad dynamic range makes assaying different pathologies easy.

Tumor Necrosis Factor-α (TNF-α) is a 17.5kDa, 157 amino acid protein that is a potent lymphoid factor, which exerts cytotoxic effects on a wide range of tumor cells and other target cells. TNF-α has been suggested to play a pro-inflammatory role and has been detected in synovial fluid of patients with rheumatoid arthritis. It is the primary mediator of immune regulation. The biosynthesis of TNF-α is tightly controlled, being produced in extremely small quantities in quiescent cells, but is a major secreted factor in activated cells.