TNF-α (human) monoclonal antibody (TNF-D)

TNFSF 2, Tumor necrosis factor-α

ELISA, Flow Cytometry, FUNC

Functional Application: selectively neutralizes human TNF-α bioactivity. TNF-D requires an incubation of antigen and antibody for 1 hour at 37 °C or 16 hours at 4 °C to completely neutralize TNF-α cytolytic activity.
Not recommended for Western blot.

TNF-D

Does not cross-react with mouse TNF-α or human or mouse TNF-β.

Liquid. In PBS.

Mouse

Recombinant human TNF-α (tumor necrosis factor-α).

IgG1

ELISA (use as capture or detection antibody in sandwich ELISA)Flow Cytometry (suitable as primary antibody for staining in FACS analysis; recommended concentration is 10µg/ml as determined on PBMC. Method: PBMC were incubated with PMA, ionomycin, and with monensin to inhibit secretion. Incubation for 20 minutes with TNF-D at 10µg/ml; stained with goat-anti-mouse IgG-FITC second step reagent for 15 minutes)Suggested dilutions/conditions may not be available for all applications.Optimal conditions must be determined individually for each application.

Human

P01375

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