TLCK

BML-PI121

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Inhibitor of trypsin and trypsin-like proteases

Irreversible inhibitor of trypsin and trypsin-like serine proteases such as granzyme D and tryptase. Also inhibits other proteases including plasmin, thrombin, and papain and some kinases including PKC. Inhibits LPS-induced expression of COX-2 in macrophages. Inhibits apoptosis (possibly through the inhibition of serine proteases). Chymotrypsin is not inhibited by TLCK, so this inhibitor can be used to block contaminating trypsin activity in chymoptrypsin preparations. TLCK can be used in combination with a chymotrypsin inhibitor to prevent proteolytic degradation during the isolation of proteins.

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Product Details

Alternative Name	Nα-Tosyl-Lys-chloromethylketone . HCl
Appearance	Off-white solid
CAS	4238-41-9
Formula	C₁₄H₂₁CIN₂O₃S . HCl
MW	369.3
Purity	≥95% (HPLC)
Sequence	Na-Tosyl-Lys-chloromethylketone
Solubility	Soluble in methanol (50mg/ml), DMSO (5mM) or water. Stock solutions at concentrations up to 10mM can be prepared in aqueous buffers with a pH of 6.0 or lower. TLCK is very unstable in buffers with a pH higher than 6 and will decompose rapidly.

Handling & Storage

Use/Stability	As indicated on product label or CoA when stored as recommended.
Long Term Storage	-20°C
Shipping	Blue Ice

Regulatory Status	RUO – Research Use Only

The small heat shock protein B8 (HSPB8) confers resistance to bortezomib by promoting autophagic removal of misfolded proteins in multiple myeloma cells: M. Hamouda, et al.; Oncotarget 5, 6252 (2014), Application(s): Analysis of velcade resistant multiple myeloma human cells by WB, Assay, Abstract — Full Text
A novel form of the RelA nuclear factor kappaB subunit is induced by and forms a complex with the proto-oncogene c-Myc: N.R. Chapman, et al.; Biochem. J. 366, 459 (2002), Abstract
Lipopolysaccharide-induced expression of cyclooxygenase-2 in mouse macrophages is inhibited by chloromethylketones and a direct inhibitor of NF-kappa B translocation: A. Abate, et al.; Prostaglandins Other Lipid Mediat. 56, 277 (1998), Abstract
15N and 1H NMR spectroscopy of the catalytic histidine in chloromethyl ketone-inhibited complexes of serine proteases: E. Tsilikounas, et al.; Biochemistry 35, 2437 (1996), Abstract
DNA degradation and proteolysis in thymocyte apoptosis: H.O. Fearnhead, et al.; Toxicol. Lett. 82/83, 135 (1995), Abstract
Granzymes, a family of serine proteases released from granules of cytolytic T lymphocytes upon T cell receptor stimulation: D.E. Jenne, et al.; Immunol. Rev. 103, 53 (1988), Abstract
Tryptase in rat mast cells: properties and inhibition by various inhibitors in comparison with chymase: M. Muramatu, et al.; Biol. Chem. Hoppe Seyler 369, 617 (1988), Abstract
N-alpha-Tosyl-L-lysine chloromethyl ketone and N-alpha-tosyl-L-phenylalanine chloromethyl ketone inhibit protein kinase C: D.H. Solomon; FEBS Lett. 190, 342 (1985), Abstract
Isolation and characterization of the histone variants in chicken erythrocytes: M.K. Urban, et al.; Biochemistry 18, 3952 (1979), Abstract
Inhibition of human thrombin assessed with different substrates and inhibitors. Characterization of fibrinopeptide binding interaction: J.J. Gorman; Biochim. Biophys. Acta. 412, 273 (1975), Abstract

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TLCK

BML-PI121

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