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TL1A expression in PBMC cell subsets from CLL patients and healthy donorsA. Quantitative real-time PCR analysis of TL1A mRNA expression in FACS-isolated cell subsets (CD3+, CD14+, and CD19+) from CLL (n = 6) and healthy donors (n = 3). Gene expression was normalized vs β-actin expression and represented as relative expression of TL1A mRNA levels with respect to mean TL1A mRNA across samples (set as 1). Lines represent median values. B. TL1A protein expression analysis in gated-cell subsets (CD3+, CD68+, CD5+/CD19+, CD19+) of PBMCs from CLL patients (n = 3) and healthy donors (n = 3). PBMCs were unstimulated or stimulated with PMA/ionomycin (PMA) in presence of brefeldin A (BFA). Dot plots are representative examples of TL1A flow cytometry analysis in gated-cell subsets (CD3+, CD68+, CD5+/CD19+, CD19+) of PBMCs from CLL patient and healthy donor. Number in bold indicate the percentage of TL1A-positive cells.
Image collected and cropped by CiteAb under a CC-BY license from the following publication: Expression and function of the TL1A/DR3 axis in chronic lymphocytic leukemia. Oncotarget (2015)
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RUO – Research Use Only |
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