TIMP-1 (human) ELISA kit

• Highly sensitive measurement of TIMP-1, detecting as little as 30 pg/ml
• Negligible cross-reactivity with similar proteins (TIMP-2, TIMP-3, TIMP-4, MMP-1, MMP-2, MMP-3, MMP-7, MMP-8, MMP-9, MMP-13)
• High throughput format with results in 2.5 hours for up to 39 samples in duplicate
• Fully quantitative results that surpass semi-quantitative Western blot analysis

The TIMP-1 ELISA kit is a colorimetric immunometric immunoassay kit with results in 2.5 hours.

Tissue inhibitor of metalloproteinases-1, TIMP-1, is one of the four members of the TIMP family which inhibit the activity of the matrix metalloproteinases (MMPs), a large group of peptidases involved in degradation of the extracellular matrix. TIMP-1 displays multiple biological functions. TIMP-1 is capable of inhibiting the activities of most MMPs except membrane-type MMP subfamily. Associated with its inhibitory activity on MMPs, TIMP-1 is able to promote cell proliferation and survival in a wide range of cell types. Recent findings have separated the MMP inhibitory activity of TIMP-1 from its growth promoting effect. TIMP-1 exhibits growth factor-like activity and acts as a cell survival factor which may be mediated through ligand-receptor interactions.

The balance of TIMPs/MMPs plays a crucial role in extracellular matrix homeostasis and remodeling, the imbalance of TIMPs/MMPs is involved in various kinds of diseases in multiple organs. Breaking the balance between MMPs and TIMPs occurs in various pathologic processes and involves all the members of these two families. Elevation of TIMP-1 has been reported in diseases such as systemic sclerosis, amyotrophic lateral sclerosis (ALS), renal disease and invasive tumors. Down regulation of TIMP-1 was found to be associated with ischemic heart failure and chronic inflammation.