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Thyroid peroxidase (TPO) is an enzyme crucial for the synthesis of thyroid hormones. It plays a key role in the iodination of tyrosine residues in thyroglobulin. Anti-TPO antibodies are often used in the diagnosis of autoimmune thyroid diseases, such as Hashimoto’s thyroiditis and Graves’ disease.
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Immunofluorescence staining of TPO in U251MG cells. Cells were fixed with 4% PFA, permeabilized with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Human TPO polyclonal antibody (dilution ratio 1:1000) at 4°C overnight. Then cells were stained with the Alexa Fluor$488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to cytoplasm.
Immunochemical staining of human TPO in human thyroid with rabbit polyclonal antibody (1:1000, formalin-fixed paraffin embedded sections).
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RUO – Research Use Only |
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