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Thrombopoietin (TPO) is a growth factor that is produced by the liver and kidney. TPO acts through the TPO receptor to promote megakaryocyte maturation and differentiation, which leads to the production of platelets.
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Figure 2: 1µg in each lane (-) non-reducing conditions and (+) reducing conditions in a 4-20% TRIS-Glycine gel, stained with Coomassie Blue. Mouse TPO has a predicted MW of 18.7 kDa.
Figure 1: Mouse TPO Bioactivity Data. Serial dilutions of mouse TPO, starting at 200ng/ml, were added to MO7e cells growing in the presence of 1ng/ml IL-3 and 2.5ng/ml SCF. Proliferation was measure after 5 days and the linear portion of the curve was us used to calculate the ED50. The ED50 for this lot of mouse TPO was less than 0.8ng/ml. This value is comparable to the typical expected range of 1ng/ml.
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RUO – Research Use Only |
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