TG2-CovTest

Specific Tissue Transglutaminase 2 (tTG/TGase2) Colorimetric Microassay

For the quantitative determination of specific TG2 crosslinking activity in purified enzyme preparations, cells and crude tissue homogenates.

Microtiter strips with covalently bound spermine
R1: DTT, 12×8-wells strip
R2: EDTA (Negative Control), 0.5 ml
R3: Reaction Buffer (biotin-pepT26/ CaCl₂), 2 vials (lyophilized powder)
R4: Enzyme Tracer (SAv-HRP), 50 µl
R5: Wash Buffer 10X, 30 ml
R6: Diluent Buffer 10X, 10 ml
R7: HRP Substrate Solution, 12 ml
R8: Blocking Reagent, 6 ml

The kit is designed to have the following advantages:

– Measurement of TG2 with low or no interference with other TGs isoforms.
– High sensitivity (equivalent to commonly used radiometric filter paper method).
– Rapid test (performance time is 30 minutes).
– Simultaneous screening of TG2 activity using 96 wells (12 X 8-well strips).
– Evaluation of TG2 inhibitors.

For the Original Manufacturer’s Data Sheet please click here.
Our product description may differ slightly from the Original Manufacturer’s Data Sheet.

For the original reference with the assays please click here.