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Testosterone high sensitivity ELISA kit

ADI-901-176

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A highly sensitive competitive immunoassay for the measurement of Testosterone in human serum and plasma samples.

Excellent sensitivity
Validated for use with human samples
Stable color-coded reagents reduce errors
Save with bulk kit package!

A highly sensitive competitive immunoassay for the measurement of Testosterone in human serum and plasma samples. Results are read on a colorimetric plate reader at 405 nm.

Testosterone is one of the most important androgens secreted into the bloodstream and is synthesized from pregnenolone which is itself formed from cholesterol. Testosterone is the main androgen secreted by the Leydig cells of the testes and effects both primary and secondary sexual development such as muscle mass and sex drive. In adult humans, testosterone circulates in plasma predominately bound to proteins, including specific sex hormone binding globulin (SHBG) and nonspecific proteins such as albumin.

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Product Details

Application	Colorimetric detection, ELISA
Application Notes	For the quantitative determination of human testosterone in plasma, serum, and urine samples.
Assay Time	3 hours
Compatibility	This product is compatible with the Absorbance 96 Plate Reader.
Contents	GxM Microtiter plate, Assay buffer, Standard, Antibody, Conjugate, Wash buffer concentrate, pNpp Substrate, Stop solution
Sensitivity	2.6 pg/ml (range 3.9 – 1000 pg/ml)
Species Reactivity	Human
Wavelength	405 nm

Handling & Storage

Short Term Storage	+4°C
Long Term Storage	+4°C
Shipping	Blue Ice

Regulatory Status	RUO – Research Use Only

Male reproductive phenotype alterations in heterozygous β-globin gene knockout thalassemia (BKO) mice as a model for β-thalassemia patients: Aiemongkot, S., Ruschadaariyachat, S., et al.; Sci. Rep. 15, 4903 (2025), Abstract
Hormonal differences in perpetrators of intimate partner violence: Cantos, A. L., Ontiveros, G., et al.; Front. Psychiatry 15, 1432864 (2024), Abstract
Ex vivo effects of bisphenol A or zearalenone on the prepubertal rat testis: M. Drouault, et al.; Environ. Toxicol. Pharmacol. 101, 104203 (2023), Abstract
Early Castration in Horses Does Not Impact Osteoarticular Metabolism: Rouge, M., Legendre, F., et al.; Int. J. Mol. Sci. 24, (2023), Abstract
Reversal of Lactate and PD-1-mediated Macrophage Immunosuppression Controls Growth of PTEN/p53-deficient Prostate Cancer: Chaudagar, K., Hieromnimon, H. M., et al.; Clin. Cancer Res. 29, 1952 (2023), Abstract
Structural and functional changes in rat uterus induced by neonatal androgenization: R.C. Genaro, et al.; J. Mol. Histol. 53, 903 (2022), Abstract
Testing hormonal responses to real and simulated social challenges in a competitive female bird: George, E. M., Wolf, S. E., et al.; Behav. Ecol. 33, 233 (2022), Abstract
Testosterone, signal coloration, and signal color perception in male zebra finch contests: Green, P. A., George, E. M., et al.; Ethology 128, 131 (2022), Abstract
Social instability influences rank-specific patterns of oxidative stress in a cichlid fish: S.E. Border, et al.; J. Exp. Biol. 224, 237172 (2021), Abstract
Tri-ortho-cresyl phosphate exposure leads to low egg production and poor eggshell quality via disrupting follicular development and shell gland function in laying hens: J. Li, et al.; Ecotoxicol. Environ. Saf. 225, 112771 (2021), Abstract