A highly sensitive competitive immunoassay for the measurement of Testosterone in human serum and plasma samples.
- Excellent sensitivity
- Validated for use with human samples
- Stable color-coded reagents reduce errors
A highly sensitive competitive immunoassay for the measurement of Testosterone in human serum and plasma samples. Results are read on a colorimetric plate reader at 405 nm.
Testosterone is one of the most important androgens secreted into the bloodstream and is synthesized from pregnenolone which is itself formed from cholesterol. Testosterone is the main androgen secreted by the Leydig cells of the testes and effects both primary and secondary sexual development such as muscle mass and sex drive. In adult humans, testosterone circulates in plasma predominately bound to proteins, including specific sex hormone binding globulin (SHBG) and nonspecific proteins such as albumin.
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Product Details
| Application |
Colorimetric detection, ELISA |
|---|---|
| Application Notes |
For the quantitative determination of human testosterone in plasma, serum, and urine samples. |
| Assay Time |
3 hours |
| Compatibility |
This product is compatible with the Absorbance 96 Plate Reader. |
| Contents |
GxM Microtiter plate, Assay buffer, Standard, Antibody, Conjugate, Wash buffer concentrate, pNpp Substrate, Stop solution |
| Sensitivity |
2.6 pg/ml (range 3.9 – 1000 pg/ml) |
| Species Reactivity |
Human |
| Wavelength |
405 nm |
Handling & Storage
| Short Term Storage |
+4°C |
|---|---|
| Long Term Storage |
+4°C |
| Shipping |
Blue Ice |
| Regulatory Status |
RUO – Research Use Only |
|---|
- The Interplay of Biomarkers and Psychosocial Variables in IPV Perpetration.: Cantos, A., Mondolfi, M. L., et al.; Behav. Sci. (Basel) 15, 1075 (2025), Abstract
- Seasonal divergence in reproductive timing on the verge of spring: comparing hypothalamic transcriptome of two seasonally sympatric North American songbird populations.: Singh, D., Fudickar, A. M., et al.; Front. Physiol. 16, 1627516 (2025), Abstract
- Oral Ingestion of Bean Sprouts Containing the HASPIN Inhibitor Coumestrol Increased Blood Testosterone Levels in Men.: Kakazu, K., Tsujimura, A., et al.; Biology (Basel) 14, 907 (2025), Abstract
- Male reproductive phenotype alterations in heterozygous β-globin gene knockout thalassemia (BKO) mice as a model for β-thalassemia patients: Aiemongkot, S., Ruschadaariyachat, S., et al.; Sci. Rep. 15, 4903 (2025), Abstract
- Hormonal differences in perpetrators of intimate partner violence: Cantos, A. L., Ontiveros, G., et al.; Front. Psychiatry 15, 1432864 (2024), Abstract
- Ex vivo effects of bisphenol A or zearalenone on the prepubertal rat testis: M. Drouault, et al.; Environ. Toxicol. Pharmacol. 101, 104203 (2023), Abstract
- Reversal of Lactate and PD-1-mediated Macrophage Immunosuppression Controls Growth of PTEN/p53-deficient Prostate Cancer: Chaudagar, K., Hieromnimon, H. M., et al.; Clin. Cancer Res. 29, 1952 (2023), Abstract
- Early Castration in Horses Does Not Impact Osteoarticular Metabolism: Rouge, M., Legendre, F., et al.; Int. J. Mol. Sci. 24, (2023), Abstract
- Structural and functional changes in rat uterus induced by neonatal androgenization: R.C. Genaro, et al.; J. Mol. Histol. 53, 903 (2022), Abstract
- Testosterone, signal coloration, and signal color perception in male zebra finch contests: Green, P. A., George, E. M., et al.; Ethology 128, 131 (2022), Abstract
- Testing hormonal responses to real and simulated social challenges in a competitive female bird: George, E. M., Wolf, S. E., et al.; Behav. Ecol. 33, 233 (2022), Abstract
- Tri-ortho-cresyl phosphate exposure leads to low egg production and poor eggshell quality via disrupting follicular development and shell gland function in laying hens: J. Li, et al.; Ecotoxicol. Environ. Saf. 225, 112771 (2021), Abstract
- Breeding at higher latitude as measured by stable isotope is associated with higher photoperiod threshold and delayed reproductive development in a songbird: D. Singh, et al.; Horm. Behav. 128, 104907 (2021), Sample Type: Bird (Dark-eyed junco [Junco hyemalis]) Plasma, Abstract
- Nesting strategy shapes territorial aggression but not testosterone: a comparative approach in female and male birds: S. Lipshutz, et al.; Horm. Behav. 133, 104995 (2021), Sample Type: Bird (Tree swallow [Tachycineta bicolor], Barn swallow [Hirundo rustica], Eastern bluebird [Sialia sialis], American robin [Turdus migratorius]) Plasma, Abstract
- Increased testosterone and proinflammatory cytokines in patients with polycystic ovary syndrome correlate with elevated GnRH receptor autoantibody activity assessed by a fluorescence resonance energy transfer-based bioassay: H. Li, et al.; Endocrine 74, 163 (2021), Abstract
- Experimental competition induces immediate and lasting effects on the neurogenome in free-living female birds: Bentz, A. B., George, E. M., et al.; PNAS 118, (2021), Abstract
- A single GnRH challenge promotes paternal care, changing nestling growth for one day: George, E. M., Navarro, D., et al.; Horm. Behav. 130, 104964 (2021), Abstract
- Testosterone and depressive symptoms during the late menopause transition: B. Sander, et al.; Biol. Sex Differ. 12, 44 (2021), Application(s): Human urine, Abstract — Full Text
- Social instability influences rank-specific patterns of oxidative stress in a cichlid fish: S.E. Border, et al.; J. Exp. Biol. 224, 237172 (2021), Abstract
- Local adaptation from afar: migratory bird populations diverge in the initiation of reproductive timing while wintering in sympatry: Wanamaker, S. M., Singh, D., et al.; Biol. Lett. 16, 20200493 (2020), Abstract
- Nesting strategy shapes territorial aggression but not testosterone: a comparative approach in female and male birds: Lipshutz, S., Rosvall, K., et al.; bioRxiv , (2020)
- Diet during early life defines testicular lipid content and sperm quality in adulthood: Crisóstomo, L., Videira, R. A., et al.; Am. J. Physiol. Endocrinol. Metab. 319, E1061 (2020), Abstract
- Testosterone secretion varies in a sex- and stage-specific manner: Insights on the regulation of competitive traits from a sex-role reversed species: Lipshutz, S. E., Rosvall, K. A., et al.; Gen. Comp. Endocrinol. 292, 113444 (2020), Abstract
- The Role of GnRH Receptor Autoantibodies in Polycystic Ovary Syndrome: Kem, D. C., Li, H., et al.; J. Endocr. Soc. 4, bvaa078 (2020), Abstract
- Breeding at higher latitude as measured by stable isotope is associated with higher photoperiod threshold and delayed reproductive development in a songbird: Singh, D., Reed, S., et al.; bioRxiv , (2019)
- Social rank, color morph, and social network metrics predict oxidative stress in a cichlid fish: S.E. Border, et al.; Behav. Ecol. 30, 490 (2019), Sample Type: Fish (Astatotilapia burtoni) Plasma, Abstract
- Testosterone production and social environment vary with breeding stage in a competitive female songbird: E.M. George, et al.; Horm. Behav. 103, 28-35 (2018), Abstract
- Dysregulation of Notch and ERα signaling in AhR-/- male mice: B. Huang, et al.; PNAS 18, 113 (2016), Application(s): ELISA using mouse serum, Abstract — Full Text
- The early evolving sex hormone environment is associated with significant outcome and inflammatory response differences after injury: S.J. Zolin, et al.; J. Trauma Acute Care Surg. 78, 451 (2015), Application(s): ELISA using human plasma, Abstract — Full Text
- Of mice and men–warning: intact versus castrated adult male mice as xenograft hosts are equivalent to hypogonadal versus abiraterone treated aging human males, respectively: J.P. Michiel Sedelaar, et al.; Prostate 73, 1316 (2013), Application(s): ELISA using mouse serum, Abstract — Full Text
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A highly sensitive competitive immunoassay for the measurement of Testosterone in human serum and plasma samples.
| Application | Colorimetric detection, ELISA |
|---|---|
| Assay Time | 3 hours |
| Sensitivity | 2.6 pg/ml (range 3.9 – 1000 pg/ml) |
| Species Reactivity | Human |
Last modified: May 29, 2024
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