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Spin trapping reagent

Spin trap, useful for both in vitro and in vivo experiments.

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Alternative Name	4-Oxo-2,2,6,6-tetramethylpiperidine-1-oxyl, 4-Oxo-TEMPO
Appearance	Orange crystalline powder.
CAS	2896-70-0
Formula	C₉H₁₆NO₂
Identity	Determined by IR.
MW	170.2
Purity	≥98% (Assay)
Solubility	Soluble in acetone, DMSO, diethylether, 100% ethanol or water.

Use/Stability	As indicated on product label or CoA when stored as recommended.
Handling	Protect from light.
Long Term Storage	-20°C
Shipping	Blue Ice

Regulatory Status	RUO – Research Use Only

Metabolism of the stable nitroxyl radical 4-oxo-2,2,6, 6-tetramethylpiperidine-N-oxyl (TEMPONE): C. Kroll & H.H. Borchert; Eur. J. Pharm. Sci. 8, 5 (1999), Abstract
Cytotoxicity of spin trapping compounds: R.F. Haseloff, et al.; FEBS Lett. 418, 73 (1997), Abstract
Characterization of the 4-oxo-2,2,6,6-tetramethylpiperidinooxy dosimeter for in situ radiolysis electron spin resonance studies: K.P. Madden; Radiat. Res. 147, 335 (1997), Abstract
Nitrone spin traps and a nitroxide antioxidant inhibit a common pathway of thymocyte apoptosis: A.F. Slater, et al.; Biochem. J. 306, 771 (1995), Abstract
Inhibition of oxygen-dependent radiation-induced damage by the nitroxide superoxide dismutase mimic, tempol: J.B. Mitchell, et al.; Arch. Biochem. Biophys. 289, 62 (1991), Abstract
Superoxide reaction with nitroxides: A. Samuni, et al.; Free Rad. Res. Commun. 9, 241 (1990), Abstract
Biologically active metal-independent superoxide dismutase mimics: J.B. Mitchell, et al.; Biochemistry 29, 2802 (1990), Abstract
Cytotoxicity of commonly used nitroxide radical spin probes: E.G. Ankel, et al.; Life Sci. 40, 495 (1987), Abstract

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