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TCP-1α monoclonal antibody (91a)

ADI-CTA-191

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TCP-1 alpha is a 60 kDa subunit of a cytosolic hetero-oligomer chaperone that is known to be involved in the folding of Actin and Tubulin. This protein is a member of the Chaperonin family, which includes E. coli GroEL, the mitochondrial Heat-shock protein Hsp60, the plastid Rubisco-subunit-binding protein, and the archaebacterial protein TF55. These Chaperonins assist the folding of proteins upon ATP hydrolysis.

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This antibody is covered by our Worry-Free Guarantee.

TCP-1α monoclonal antibody (91a) Flow Cytometry — Flow cytometric analysis human colon cancer Coca-2 cells were analyzed by FLOW cytometry using isotype control antibody (left) or TCP-1alpha antibody, clone #91a (right).

TCP-1α monoclonal antibody (91a) Western blot — Western blot analysis of CTA-191: Lane 1: MW marker, Lane 2: HeLa Cell Lysate, Heat shocked, Lane 3: PC-12 Cell Lysate Heat Shocked, Lane 4: 3T3 Cell Lysate, Heat Shocked, Lane 5, RK-13 Cell Lysate, Heat Shocked.

Product Details

Alternative Name	CCT, p63, Tric, T-complex 1
Application	Flow Cytometry, ICC, IHC, IP, WB
Application Notes	Detects a band of ~60kDa by Western blot.
Clone	91a
Formulation	Liquid. In PBS containing 50% glycerol and 0.09% sodium azide.
GenBank ID	D90344
Host	Rat
Immunogen	Recombinant mouse TCP-1α (C-terminal fragment).
Isotype	IgG2a
Purity Detail	Protein G affinity purified.
Recommendation Dilutions/Conditions	Flow Cytometry (1:100)Western Blot (1:1,000, colorimetric)Suggested dilutions/conditions may not be available for all applications.Optimal conditions must be determined individually for each application.
Source	Purified from mouse ascites.
Species Reactivity	Bovine, C. elegans, Dog, Drosophila, Guinea pig, Hamster, Human, Monkey, Mouse, Plant, Porcine, Rabbit, Rat, Yeast
Technical Info / Product Notes	Recommended by the Human Protein Atlas Organization for IHC (Ensembl No. ENSG00000120438).
UniProt ID	P11983
Worry-free Guarantee	This antibody is covered by our Worry-Free Guarantee.

Handling & Storage

Handling	Avoid freeze/thaw cycles.
Long Term Storage	-20°C
Shipping	Blue Ice

Regulatory Status	RUO – Research Use Only

Proteomic identification of protein targets for 15-deoxy-Δ(12,14)-prostaglandin J2 in neuronal plasma membrane.: Yamamoto, Y., Takase, K., et al.; PLoS One 6, e17552 (2011), Application(s): WB, Abstract
A 102 kDa subunit of a Golgi-associated particle has homology to beta subunits of trimeric G proteins: K.R. Willison, et al.; EMBO J. 12, 2847 (1993), Application(s): IP using human samples, Abstract
T-complex polypeptide-1 is a subunit of a heteromeric particle in the eukaryotic cytosol: K. Willison, et al.; Nature 358, 249 (1992), Application(s): IP (M), ICC (MO) using mouse & monkey samples, Abstract