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Synaptotagmin monoclonal antibody (ASV30)

ADI-SYA-130

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Synaptotagmins (Syt) are membrane-trafficking proteins characterized by an N-terminal transmembrane region, a variable linker, and two C-terminal C2-domains, C2A and C2B. At least twelve Synaptotagamins are expressed in vertebrates and can be found in both vesicular and plasma membranes.

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Synaptotagmin monoclonal antibody (ASV30) Western blot — Western blot analysis of Synaptotagmin: Lane 1: MW marker, Lane 2: Mouse Brain Tissue Lysate (Prod No. ADI-LYT-MB100), Lane 3: Rat Brain Tissue Lysate (Prod No. ADI-LYT-RB100).

Product Details

Alternative Name	SYT
Application	IHC, WB
Application Notes	Detects a band of ~65kDa by Western blot.
Clone	ASV30
Formulation	Liquid. In PBS containing 50% glycerol and 0.09% sodium azide.
GenBank ID	X52772
Host	Mouse
Immunogen	Rat brain synaptic junction protein complexes .
Isotype	IgG2a
Purity Detail	Protein G affinity purified.
Recommendation Dilutions/Conditions	Western Blot (1:1,000, colorimetric)Suggested dilutions/conditions may not be available for all applications.Optimal conditions must be determined individually for each application.
Source	Purified from mouse ascites.
Species Reactivity	Human, Mouse, Rat
UniProt ID	P21707
Worry-free Guarantee	This antibody is covered by our Worry-Free Guarantee.

Handling & Storage

Handling	Avoid freeze/thaw cycles.
Long Term Storage	-20°C
Shipping	Blue Ice

Regulatory Status	RUO – Research Use Only

An ultra-stable cytoplasmic antibody engineered for in vivo applications: H. Kabayama, et al.; Nat. Commun. 11, 336 (2020), Abstract — Full Text
Botulinum neurotoxin A activity is dependent upon the presence of specific gangliosides in neuroblastoma cells expressing synaptotagmin I: C.L. Schengrund, et al.; J. Biol. Chem. 277, 32815 (2002), Application(s): WB using human & mouse samples, Abstract
Identification of a synaptic vesicle-specific membrane protein with a wide distribution in neuronal and neurosecterory tissue: W.D. Matthew, et al.; J. Cell Biol. 91, 257 (1981), Application(s): WB, IHC using rat samples, Abstract