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- Increased stability
- Enhanced immune activation
SUPERKILLERTRAIL® (soluble) (human), (recombinant) is an enhanced ligand that utilizes the Killer™ linker peptide mutated to increase disulfide-mediated cross-linking to form a more stable oligomer.
It has been well established that signals transmitted via TNF ligands and receptors are critical in mediating inflammation, cancer, and immunity. This has led to drug development aiming to either reduce or enhance receptor response. One limitation in this process is the inherent instability of recombinant ligands and their inability to effectively mimic the natural clustering of TNF receptors on the cell surface required for immune activation. Oligomerization of TNF ligands such as SUPERKILLERTRAIL® has been shown to improve stability and significantly enhance immune activation compared to recombinant ligands alone.
TNF-related apoptosis-inducing ligand (TRAIL) is a protein ligand that induces the process of cell death called apoptosis.
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Figure: Apoptosis of TRAIL-sensitive cells. Apoptosis induction in BJAB cells by SUPERKILLERTRAIL®, Soluble (human) (rec.) reveals killing activity at concentrations of 10-15ng/ml, on Jurkat cells even as low as 1-10ng/ml. Method: 5×104 human BJAB cells were cultured in 50µl RPMI 1640 medium containing 10% fetal calf serum for 18 hours at 37°C in a 96-well plate in the presence of the indicated concentrations of SUPERKILLERTRAIL®, Soluble (human) (rec.). Concentrations of SUPERKILLERTRAIL®, Soluble (human) (rec.) required to induce apoptosis may vary depending on the cell type studied. Cell death was quantified by forward/sideward scatter (FSC/SSC) combined with propidium iodide (PI) staining analyzed by flow cytometry.
Figure: Comparison of pro-apoptotic activities of human and mouse SUPERKILLERTRAIL®, Soluble (human) (rec.) on human tumor cell lines Jurkat and CEM.Method: Cells were treated with mouse and human SUPERKILLERTRAIL®, Soluble (human) (rec.) (CC-mutant) (50ng/ml) for 14 hours, stained with propidium iodide and analyzed by flow cytometry.
| Regulatory Status |
RUO – Research Use Only |
|---|
Related Products
KillerTRAIL™ Protein (soluble) (human), (recombinant)
ALX-201-073
Enhanced ligand that utilizes the proprietary Killer™ linker peptide that promotes stability.
| Alternative Name | Apo-2L, TNFSF 10, CD253 |
|---|---|
| Purity | ≥99% (SDS-PAGE, MS analysis) |
| Source | Produced in E. coli. The extracellular domain of human TRAIL (aa 95-281) is fused at the N-terminus to a His-tag and a linker peptide. |
| Alternative Name | Apo-2L, TNFSF 10, CD253 |
|---|---|
| Purity | ≥98% (SDS-PAGE) |
| Source | Produced in E. coli. The extracellular domain of human TRAIL (aa 95-281) is fused at the N-terminus to a His-tag and a linker peptide. |
Last modified: May 29, 2024